1. Construction of clone-based physical maps suitable for sequencing the human genome. The first-generation physical maps of human chromosomes constructed in the Human Genome Project are predominantly yeast artificial chromosome (YAC)-based sequence-tagged site (STS)-content maps, which provide both collections of overlapping YAC clones and ordered sets of STSs. For various technical reasons, YACs are not ideal templates for DNA sequencing. Instead, the mapped STSs can be used to isolate smaller-insert bacterial artificial chromosome (BAC) (or P1-derived artificial chromosome [PAC]) clones, which in turn can be analyzed124, 125, 272 and assembled into contigs. Typically, the resulting nascent BAC contigs are smaller than the corresponding YAC contig. However, the gaps between BAC contigs can be filled by various methods (e.g., by developing new STSs from appropriate BAC ends and then isolating additional overlapping clones). From the resulting BAC contigs, minimally overlapping BACs (shown as dotted lines) can be selected and individually subjected to shotgun sequencing (see the text and Fig ).
Source: The Human Genome Project and Its Impact on the Study of Human Disease, The Online Metabolic and Molecular Bases of Inherited Disease
Citation: Valle D, Beaudet AL, Vogelstein B, Kinzler KW, Antonarakis SE, Ballabio A, Gibson K, Mitchell G. The Online Metabolic and Molecular Bases of Inherited Disease; 2014 Available at: Accessed: December 21, 2017
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