1. How C. elegans embryos divide?
What is the major issue of this paper?
How C. elegans embryos divide?
Whether gut differentiation is dependent on inductive signals?
How to separate C. elegans blastomeres?
How to observe gut differentiation
Whether gut differentiation is dependent on local determinants?

2. What is the major approach that the author used to address his major question?
Antibody staining
Separation and reconstitution of blastomeres
C. elegans lineage analysis
Multiple approaches are used and needed.
Genetic analysis

3. How did the author argue against the possibility that separating blastomeres from each other early in the cell cycle may damage the ability of EMS to differentiate into gut?
Citing published literatures showing that this isolation procedure is safe and never affects the differentiation potential of isolated blastomeres
Showing that in unoperated embryos EMS sometimes failed to differentiate into a gut
Showing that damaging EMS or its neighboring blastomeres did not affect gut differentiation
Putting isolated blastomeres back and showing that gut differentiated normally in all cases.
The author avoided to discuss this issue

4. Which of the following is important for gut differentiation in C
Which of the following is important for gut differentiation in C. elegans?
ABa
ABa + ABp
ABp P2
ABp + P2

5. Which of the following is a correct statement?
Cytoplasmic determinant is not important for gut differentiation
The interaction between EMS and P2 occurs soon after cell division
Recombining isolated EMS and P2 always results in gut differentiation
Recombining isolated EMS and ABx always never yields correct gut differentiation
All of the above

6. What is the most important lesson that you learn from reading this paper?
It is a very cool paper
Inductive signal is important for C. elegans gut differentiation
One can always question existing or established dogma
C. elegans is a good animal model for studying embryo development
You need to have very good hands to be a good scientist