1. HPLC Bulk Property Detectors
Refractive Index Detector

2. HPLC Bulk Property Detectors
Refractive Index Detector
Plus:
1) Measures a bulk property
2) Nearly Universal (different RI than mobile phase)
3) Comparable response for different analytes
4) Detects species with no chromophores
Minus:
1) Temperature dependent
2) Poor sensitivity (LOD ≈ 100 ng)
3) No gradient elution

3. HPLC Bulk Property Detectors
Evaporative Light Scattering Detector
Solid state laser

4. HPLC Bulk Property Detectors
Evaporative Light Scattering Detector
Plus:
1) Measures a bulk property
2) Nearly Universal (must be non-volatile)
3) Does not detect liquids (gradients are ok)
4) Detects species with no chromophores
Minus:
1) Signal not linear with concentration
2) Fair sensitivity (LOD ≈ 1 ng)
3) No salts or buffers in mobile phase

5. HPLC Bulk Property Detectors
Conductivity Detector

6. HPLC Bulk Property Detectors
Conductivity Detector
Plus:
1) Measures a bulk property
2) Nearly Universal (must be ionic)
3) Common for Ion Exchange LC
4) Detects species with no chromophores
5) Simple, robust
Minus:
1) Fair sensitivity (LOD ≈ 1 ng)
2) No salts or buffers in mobile phase
3) Gradients a challenge

7. HPLC Specific Property Detectors
Conventional UV-Vis Absorption Detector
Photodiode 1
Photodiode 2
Hg Pen
Lamp
254 nm

8. HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
Deuterium
Lamp
Photodiode
Array

9. HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector

10. HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
Plus:
1) Measures a specific property
2) Nearly Universal (must have chromophore)
3) Most common of all detectors (~75%)
4) Potential to provide qualitative info.
5) Simple, robust
Minus:
1) Fair sensitivity (LOD ≈ 1 ng)
2) Expensive with PDA, limited with Hg Lamp
3) Misses some important analytes

11. HPLC Specific Property Detectors
Fluorescence Detector

12. HPLC Specific Property Detectors
Fluorescence Detector

14. HPLC Specific Property Detectors
Fluorescence Detector
Plus:
1) Measures a specific property
2) Highly Selective (must fluoresce)
3) Second most common of all detectors (~15%)
4) High sensitivity (LOD ≈ 0.01 ng)
5) Can interrogate very small volumes
Minus:
1) Not Universal
2) Limited Applications

15. HPLC Specific Property Detectors
Electrochemical Detector

16. HPLC Specific Property Detectors
Electrochemical Detector
Plus:
1) Measures a specific property
2) Highly Selective (depends on reduction potential)
3) High sensitivity (LOD ≈ 0.01 ng)
Minus:
1) Not Universal
2) Must have electrolyte in mobile phase
3) Mobile phase must be aqueous
4) Gradients not possible

17. HPLC Element Specific Detectors
ICP Detector Detector

18. HPLC Element Specific Detectors
ICP Detector Detector
Plus:
1) Can be Highly Selective
2) Can be nearly Universal
3) Can have single sensitivity for all analytes
Minus:
1) $$$
2) Mobile phase must be aqueous
4) Mobile phase must not contain element of interest

19. UV-Vis detection of sugars in juice

20. UV-Vis detection of sugars in juice

21. ICP Carbon detection of sugars in juice

22. ICP Carbon detection of sugars in orange juice

23. ICP Carbon detection of amino acids

24. ICP Carbon detection of amino acids

26. HPLC Derivatization Detectors
Tag analytes with fluorophore
Before or After Separation

27. HPLC Derivatization Detectors
Tag analytes with fluorophore
Before or After Separation

28. Detect the change in a constant signal
HPLC Indirect Detectors
Detect the change in a constant signal
as the analyte passes

29. Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector

30. Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector

31. Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector

32. Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector

33. Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector

34. Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector