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Amplification and Deletion of Topoisomerase IIα Associate with ErbB-2 Amplification and Affect Sensitivity to Topoisomerase II Inhibitor Doxorubicin in.

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Presentation on theme: "Amplification and Deletion of Topoisomerase IIα Associate with ErbB-2 Amplification and Affect Sensitivity to Topoisomerase II Inhibitor Doxorubicin in."— Presentation transcript:

1 Amplification and Deletion of Topoisomerase IIα Associate with ErbB-2 Amplification and Affect Sensitivity to Topoisomerase II Inhibitor Doxorubicin in Breast Cancer  Tero A.H. Järvinen, Minna Tanner, Virpi Rantanen, Maarit Bärlund, Åke Borg, Seija Grénman, Jorma Isola  The American Journal of Pathology  Volume 156, Issue 3, Pages (March 2000) DOI: /S (10) Copyright © 2000 American Society for Investigative Pathology Terms and Conditions

2 Figure 1 Copy number aberrations of ErbB-2 and topoisomerase IIα in breast cancer. ErbB-2 amplification in cell lines BT-474, MDA-361, and UACC-812 (A−C. was associated with no change, deletion or amplification of topoIIα gene (relative to 17 centromere, D−F, respectively). G and H: Clinical breast tumors with ErbB-2 and topoIIα amplification. I: Physical deletion of topoisomerase IIα gene in primary breast tumor. The fluorochrome colors used for each probe are indicated in each panel. DAPI (blue) was used as counterstain. Note that not all signals demonstrating amplified gene copies are in the same focal plane. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology Terms and Conditions

3 Figure 2 Protein expression of topoisomerase IIα and cyclin B1 in five breast cancer cell lines by Western blotting. The quantitated band intensities of topo IIα were adjusted by those of cyclin B1 to eliminate the differences in proliferation rates of the breast cancer cell lines (see Table 1). Even without any adjustments, strong immunoreactive bands can be seen in cell lines UACC-812 and SK-BR-3 (topoIIα amplification in both cell lines), and the weakest bands in MDA-361 (with topoIIα deletion). High-level topoisomerase IIα expression in five primary breast carcinomas with amplification of topoisomerase IIα (lanes 1–5) compared with two primary tumors with normal copy number of topoisomerase IIα (lanes 6 and 7). The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology Terms and Conditions

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