1. Charles Benson Olson Lab Group Meeting July 14th, 2017
Olson Research Group
University of California, Davis

2. Why do we care? Post-traumatic stress disorder (PTSD):
Chronic and debilitating
7.8% life-time prevalence
More likely in trauma-exposed patients
Symptoms:
Persistent re-experiencing of memories
Avoidance of cues/situations
Emotional numbing
Hyperarousal
Olson Research Group
University of California, Davis

3. Pavlovian Fear Conditioning and Extinction
Thorpe, L. (2014). Reconciling the past with pills. Knowing neurons. Retrieved from
Olson Research Group
University of California, Davis

4. Pavlovian Fear Conditioning and Extinction
Flores, Á., Saravia, R., Maldonado, R., & Berrendero, F. (2015). Orexins and fear: Implications for the treatment of anxiety disorders. Trends in Neurosciences, 38(9), 550–559.
Olson Research Group
University of California, Davis

5. Clearing the Air: mTOR
mTOR – mammalian/mechanistic Target of Rapamycin
mTOR kinase structure
Rapamycin
mTOR downstream signaling pathway
Johnson, S. C., Rabinovitch, P. S., & Kaeberlein, M. (2013). mTOR is a key modulator of ageing and age-related disease. Nature, 493(7432), 338–45.
Olson Research Group
University of California, Davis

6. Clearing the Air: Intracellular Signaling Cascades
PIP3/Akt/mTOR Pathway
Serine/threonine kinase mechanism
Duran, A., Amanchy, R., Linares, J. F., Joshi, J., Abu-Baker, S., Porollo, A., … Diaz-Meco, M. T. (2011). P62 Is a Key Regulator of Nutrient Sensing in the mTORC1 Pathway. Molecular Cell, 44(1), 134–146.
Olson Research Group
University of California, Davis

7. mTOR’s Role in Plasticity
Duman, R. S., Aghajanian, G. K., Sanacora, G., & Krystal, J. H. (2016). Synaptic plasticity and depression: new insights from stress and rapid-acting antidepressants. Nature Medicine, 22(3), 238–249.
Olson Research Group
University of California, Davis

8. Ketamine Facilitates Cued Fear Extinction Learning
Olson Research Group
University of California, Davis

9. Ketamine Reduces Fear Recovery
Olson Research Group
University of California, Davis

10. Ketamine Increases mTOR Signaling
Olson Research Group
University of California, Davis

11. Ketamine-facilitated Fear Extinction is mTOR-dependent
Olson Research Group
University of California, Davis

12. Ketamine-facilitated Fear Extinction is mTOR-dependent
Olson Research Group
University of California, Davis

13. Influence of Ketamine on cFos Expression
Olson Research Group
University of California, Davis

14. Ketamine-facilitated Fear Extinction is AMPAR-dependent
Olson Research Group
University of California, Davis

15. Thanks for listening! Any questions? Olson Research Group
University of California, Davis