1. Bub1/BubR1 and SAC
강재승
April 25th

2. Bub1/BubR1 and SAC
Introduction

3. Spindle Assembly Checkpoint
Checks if two microtubules are correctly bound to centromere.
If SAC does not work properly, aneuploidy occurs in high rate.
Bub1 and BubR1 are parts of SAC

4. Spindle Assembly Checkpoint

5. Bub1 mutation & Cancer

6. BubR1 mutation & Cancer

7. Bub1/BubR1 and SAC
Functions of BUB1/BUBR1

8. Finding of Bub1: Yeast mutant
Budding Uninhibited by Benzimidazole
Benzimidazole: spindle assembly inhibitor

9. Finding of Bub1: Yeast mutant

10. Finding of BubR1: Bub-related

11. Function of Bub1/BubR1

12. Function of Bub1/BubR1

13. Blinkin: Bub1-recruting protein

14. Blinkin: Bub1-recruting protein

15. Blinkin: Bub1-recruting protein

16. APC/C Regulation by BubR1

17. APC/C Regulation by BubR1
APC/C: anaphase promoting complex
Ubiquitin ligase
Degrades securin/Activates seperase
Seperase cleaves cohesin
Low APC/C activity in hBubR1 fraction

18. APC/C Regulation by BubR1
Ubiquitination activity

19. APC/C, BubR1 and MCC MCC: Mitotic checkpoint complex
Protein complex consists of:
BubR1
Bub3
CDC20
MAD2

20. Sum.: Role of Bub1/BubR1 in SAC

21. Bub1/BubR1 and chromosome
Chromosome congression is interrupted by Bub1/BubR1 knockdown.

22. Bub1/BubR1 and SAC
Current issues

23. Structure of Bub1/BubR1

24. Structure of Bub1/BubR1

25. Structure of Bub1/BubR1

26. Controversy: Bub1/BubR1 as kinase
Kinase domain
Microtubule attachment vs. Chromosome congression
Highly important for sac vs. auxillary

27. Future research topics
Chromosome congression mechanism
Bub1/BubR1-like proteins and SAC
Ex) Neuron progenitor cell migration

28. Thank you for listening!