1. The paracellular permeability of opossum kidney cells, a proximal tubule cell line 
Mingyu Liang, Carla R. Ramsey, Franklyn G. Knox 
Kidney International 
Volume 56, Issue 6, Pages (December 1999)
DOI: /j x
Copyright © 1999 International Society of Nephrology Terms and Conditions

2. Figure 1
Changes of paracellular permeability after seeding. Cells were seeded on permeable Transwell supports at the indicated densities, and the paracellular permeability coefficient (Papp) for 3H-mannitol was measured at 6, 10, 24, and 48 hours and then every other day after seeding. The measurements were performed at 22°C (N = at least 4 for each time point). Symbols are: (•) OK, 5.0 × 105/cm2; (○) OK, 2.5 × 105/cm2; (▾) LLC-PK1, 2.5 × 105/cm2.
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

3. Figure 2
Effect of phorbol 12-myristate 13-acetate (PMA) on the Papp of OK and LLC-PK1 cells. Cells on Transwell supports were incubated in the regular cell culture incubator with culture medium containing 0.1 μM PMA for the indicated time before the Papp for 3H-mannitol was measured at 37°C. Symbols are: (□) control;() PMA 0.1 μm, 1.5 hr; () PMA 0.1 μM, 5 hr; *P < 0.05 vs. control; #P < 0.05 vs. 1.5 hours (N = 5 for OK cells and 4 for LLC-PK1 cells).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

4. Figure 2
Effect of phorbol 12-myristate 13-acetate (PMA) on the Papp of OK and LLC-PK1 cells. Cells on Transwell supports were incubated in the regular cell culture incubator with culture medium containing 0.1 μM PMA for the indicated time before the Papp for 3H-mannitol was measured at 37°C. Symbols are: (□) control;() PMA 0.1 μm, 1.5 hr; () PMA 0.1 μM, 5 hr; *P < 0.05 vs. control; #P < 0.05 vs. 1.5 hours (N = 5 for OK cells and 4 for LLC-PK1 cells).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

5. Figure 2
Effect of phorbol 12-myristate 13-acetate (PMA) on the Papp of OK and LLC-PK1 cells. Cells on Transwell supports were incubated in the regular cell culture incubator with culture medium containing 0.1 μM PMA for the indicated time before the Papp for 3H-mannitol was measured at 37°C. Symbols are: (□) control;() PMA 0.1 μm, 1.5 hr; () PMA 0.1 μM, 5 hr; *P < 0.05 vs. control; #P < 0.05 vs. 1.5 hours (N = 5 for OK cells and 4 for LLC-PK1 cells).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

6. Figure 3
Response of the Papp of OK cell sheets to Ca2+ switch. Ca2+ switch was performed as described in the Methods section. The Papp for 3H-mannitol was measured at 37°C. Ca2+ free = PBSA/1mM EDTA for one hour; Ca2+ restoration = returned to transport medium (containing 1.8mM Ca2+) for five hours (N = 4 to 6).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions