1. CLARITY for High-resolution Imaging and Quantification of Vasculature in the Whole Mouse Brain
Zhang Lin-Yuan 1 ;Lin #cod#x0002A;Pan 2 ;Pan Jiaji 3 ;Ma Yuanyuan 1 ;Wei Zhenyu 4 ;Jiang Lu 3 ;Wang Liping 1 ;Song Yaying 1 ;Wang Yongting 3 ;Zhang Zhijun 3 ;Jin Kunlin 5 ;Wang Qian 2 ;Yang Guo-Yuan 1, 3 ;
1 Department of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China ; 2 Medical Image Computing Lab and ; 3 Neuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai , China ; 4 Shanghai Ninth People#cod#x02019;s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai , China ; 5 Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, TX76107, USA ;
Figure 4. Imaging of vasculature in a 1-mm-thick mouse brain slice stained with lectin and claudin-5. A 3D rendering of a 1-mm-thick wild-type C57BL6J mouse brain slice stained with lectin-FITC. Images were obtained from confocal microscopy equipped with a 16#cod#x000D7; objective. The imaging volume was 4300 #cod#x003BC;m#cod#x000D7;5260 #cod#x003BC;m#cod#x000D7;880 #cod#x003BC;m, with a voxel size of 0.99 #cod#x003BC;m#cod#x000D7;0.99 #cod#x003BC;m#cod#x000D7;1.00 #cod#x003BC;m. Scale bar=1 mm. B Magnification of the white box region in A. The imaging volume was 1367 #cod#x003BC;m#cod#x000D7;682 #cod#x003BC;m#cod#x000D7;678 #cod#x003BC;m. C-E Images at different depths of the brain slice from B are shown 125 #cod#x003BC;m, 340 #cod#x003BC;m, and 580 #cod#x003BC;m relative to the top imaging surface using a 16#cod#x000D7; water-immersion objective with a voxel size of 0.99 #cod#x003BC;m#cod#x000D7;0.99 #cod#x003BC;m#cod#x000D7;1.00 #cod#x003BC;m. Scale bars=50 #cod#x003BC;m. F 3D rendering of a 1-mm-thick C57BL6J mouse brain slice stained with claudin-5 antibody. Images were obtained using confocal microscopy equipped with a 16#cod#x000D7; objective. The imaging volume was 504 #cod#x003BC;m#cod#x000D7;504 #cod#x003BC;m#cod#x000D7;900 #cod#x003BC;m, with a voxel size of 0.99 #cod#x003BC;m#cod#x000D7;0.99 #cod#x003BC;m#cod#x000D7;1.00 #cod#x003BC;m. G Magnification of white box region in F. The imaging volume was 195 #cod#x003BC;m#cod#x000D7;195 #cod#x003BC;m#cod#x000D7;100 #cod#x003BC;m, with a voxel size of 0.99 #cod#x003BC;m#cod#x000D7;0.99 #cod#x003BC;m#cod#x000D7;1.00 #cod#x003BC;m.
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