1. Creative Filtration and Chromatography Products
Luknova, Inc.
20 Cabot Blvd., Suite 300, Mansfield, MA 02048, USA
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2. About Luknova Incorporated in Princeton, New Jersey (Feb. 2004)
R&D started from the Advanced Technology & Manufacturing Center (ATMC), University of Massachusetts, Dartmouth
Relocated to Boston (2009)
Designed in Massachusetts by Luknova
Manufactured in both U.S. and overseas.
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3. Products - EasyFil™ filters
Absolutely leak free design with ground joint – built-in peace of mind in filtration
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4. Products - EasyFil™ filters
Three welded-seals and one bump seal - absolutely leak-free
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5. Products - EasyFil™ filters
Proprietary products (patents spending)
All made with polymer
Safe, not fragile
Leak-free
Faster filtration
High yield
Disposable
Reusable
Adjustable long stem - No splashing and No molecular damage
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6. Products - EasyFil™ filters
Application advantages
Speed, faster separation
Save time, save solvent
No contamination, disposable
No bypass leakage
No sample-loss to strong vacuum
No splashing
No molecular damage
Adjustable long- and short-stem
Reusable (>20 times)
Construction Characs
Polypropylene (PP) housing
Polyethylene (PE) frit (pore size 10µm)
Multiple welded-seals to prevent leakage
All parts are welded together as one piece
Recommended max temperature 110oC
14/20, 14/35, 24/25, and 24/40 standard joints
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7. Products - EasyFil™ filters
Solvent test
We tested a variety of common solvents and found there are no apparent changes at following conditions:
at the boiling points of Ethyl acetate , DMF, DMA, diethyl ether, dichloromethane, THF, hexane, and water;
at concentrated H2SO4, HCl, HBr, HI, acetic acid, and TFA;
at saturated NaOH and KOH solution;
DMSO, at temperature of 120oC (not tested above 120oC)
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8. Flash columns
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9. Flash Columns – Design Five piece composition a perforated cap
a sealing insert
an inlet filter
an outlet filter
a column body
Features
Easy assembly and disassembly
Easy sample recovery and load
Easy sorbent packing/repacking
Disposable and reusable
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10. Flash Columns – Performance
Date: March 6, 2009
Column: Luknova 40g silica
Gradient: 0 to 50% EA/Hexane
Run length: 15 min
Detection wavelength: 254 nm
Monitor wavelength: 280 nm
Flow rate: 40 mL/min
Equilibration volume: 235 mL
Initial waste: 0 mL
Column: Luknova 40g prepacked standard flash column.
Sample: A total volume 390 µL of 1:1:1 ratio of toluene, phthalic acid dimethyl ester, and phthalic acid diethyl ester mixed with 500µL methylene chloride.
Solvent: Hexane (A) and Ethyl Acetate (B).
Instrument: CombiFlash system.
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11. Performance – Luknova vs. ISCO
Luknova column did reach baseline separation
Isco RF column
ISCO column did not reach baseline separation
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12. Products – QC Control Luknova’s 5-Component QC Standard
Accurate Results
Quicker
More efficient
Symmetric peaks
Sharper peaks
No fronting
No doubling
No overlapping
High yield
No compound decomposition
Reliable purification
Consistent performance
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13. A variety of flash columns
Silica columns
4g-1500g
50 and 25µm particles
Specialty columns
Amine, Cyano, Diol, Florisil, Alumina (basic, neutral, acid)
C4, C8, C18, C30, Cyclohexyl, Phenyl
WCX, SCX, SCX-2, SAX, and WAX
Mixed phases
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