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Figure 1. MS<sup>E</sup> data of THC-COOH

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1 Figure 1. MS<sup>E</sup> data of THC-COOH
Figure 1. MS<sup>E</sup> data of THC-COOH. Channels 1 and 2 are total ion chromatograms of low and high energy scans, respectively. A and B are averaged mass spectra of peaks at 1.86 min in the Channel 1 and 2, respectively. From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

2 Figure 2. Target enhancement: the optimization of THC-COOH product ions. Peak signal intensity of product ions varies with different enhanced targets in the TOF analyzer. Black circles indicate intensity of m/z and white circles indicate intensity of m/z From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

3 Figure 3. Target enhancement: the optimization of methamphetamine product ions. Peak signal intensity of product ions varies with different enhanced targets in the TOF analyzer. Black triangles indicate intensity of m/z and white triangles indicate intensity of m/z From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

4 Figure 4. Cumulative calibration curves for THC-COOH in urine obtained by four modes of Q-TOF-MS. Six point calibrators were tested five times at each concentration. X-axis indicates expected concentration and y-axis indicates measured concentration. From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

5 Figure 5. Extended calibration curves (polynomial fit) for THC-COOH in urine obtained by four Q-TOF-MS and LC–MS-MS over a range of 5–2,000 ng/mL. X-axis indicates expected concentration and y-axis indicates measured concentration. From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

6 Figure 6. Plot A: extended calibration curves for methamphetamine in urine obtained by four Q-TOF-MS modes and LC–MS-MS over a range of 100–10,000 ng/mL. X-axis indicates expected concentration and y-axis indicates measured concentration. Plots B–E: cumulative calibration curves for methamphetamine in urine obtained by four modes of TOF-MS. Four point calibrators were tested five times at each concentration. X-axis indicates expected concentration and y-axis indicates measured concentration. From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

7 Figure 7. Peak signal intensity (A) and S/N ratio (B) for THC-COOH calibrators in four Q-TOF-MS modes. Each point indicates the mean value of five replicates. Diamond, MSE mode; circle, Q-TOF-TGT mode; triangle, Q-TOF-En-TGT mode; square, Q-TOF-En-FS. From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

8 Figure 8. Patient correlation between two Q-TOF-MS modes and LC–MS-MS
Figure 8. Patient correlation between two Q-TOF-MS modes and LC–MS-MS. Comparison of THC-COOH values measured by TOF (MS<sup>E</sup> mode and Q-TOF-En-TGT mode) and LC–MS-MS (A), and percent difference (B). From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

9 Figure 9. Patient (n = 61) correlation between four Q-TOF-MS modes and LC–MS-MS. Comparison of methamphetamine values measured by Q-TOF-MS (Q-TOF-En-FS, MS<sup>E</sup>, Q-TOF-En-TGT and Q-TOF-TGT modes) and LC–MS-MS. From: Comparison of Different Time of Flight-Mass Spectrometry Modes for Small Molecule Quantitative Analysis J Anal Toxicol. 2015;39(9): doi: /jat/bkv057 J Anal Toxicol | © The Author Published by Oxford University Press. All rights reserved. For Permissions, please

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