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Figure 1. Extraction efficiencies of COOHIbu and 2-OHIbu stereoisomers on: PDMS/DVB fiber coating (white); CW/TPR fiber coatings (black) (38). From: Recent.

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Presentation on theme: "Figure 1. Extraction efficiencies of COOHIbu and 2-OHIbu stereoisomers on: PDMS/DVB fiber coating (white); CW/TPR fiber coatings (black) (38). From: Recent."— Presentation transcript:

1 Figure 1. Extraction efficiencies of COOHIbu and 2-OHIbu stereoisomers on: PDMS/DVB fiber coating (white); CW/TPR fiber coatings (black) (38). From: Recent Advances in SPE–Chiral-HPLC Methods for Enantiomeric Separation of Chiral Drugs in Biological Samples J Chromatogr Sci. 2013;51(7): doi: /chromsci/bms262 J Chromatogr Sci | © The Author [2013]. Published by Oxford University Press. All rights reserved. For Permissions, please

2 Figure 2. Extraction optimization of (2'S,2R)-COOHIbu (asterisks), (2'R,2S)-COOHIbu (black triangles), (2'S,2S)-COOHIbu (circles), (2'R,2R)-COOHIbu (diamonds), (+)-(S)-2-OHIbu (white triangles) and (–)-(R)-2-OHIbu (squares). Effects of: time (A); concentration of NaCl (B); temperature (C); pH (D) (38). From: Recent Advances in SPE–Chiral-HPLC Methods for Enantiomeric Separation of Chiral Drugs in Biological Samples J Chromatogr Sci. 2013;51(7): doi: /chromsci/bms262 J Chromatogr Sci | © The Author [2013]. Published by Oxford University Press. All rights reserved. For Permissions, please

3 Figure 3. Desorption time by using methanol for (2'S,2R)-COOHIbu (asterisks), (2'R,2S)-COOHIbu (black triangles), (2'S,2S)-COOHIbu (circles), (2'R,2R)-COOHIbu (diamonds), (+)-(S)-2-OHIbu (white triangles) and (–)-(R)-2-OHIbu (black triangles) (38). From: Recent Advances in SPE–Chiral-HPLC Methods for Enantiomeric Separation of Chiral Drugs in Biological Samples J Chromatogr Sci. 2013;51(7): doi: /chromsci/bms262 J Chromatogr Sci | © The Author [2013]. Published by Oxford University Press. All rights reserved. For Permissions, please

4 Figure 4. Chromatograms of the enantiomeric resolution of chromakalim in human plasma by using a Chiralpak AD-R column with mobile phase of water–acetonitrile (70:30, v/v) (30). From: Recent Advances in SPE–Chiral-HPLC Methods for Enantiomeric Separation of Chiral Drugs in Biological Samples J Chromatogr Sci. 2013;51(7): doi: /chromsci/bms262 J Chromatogr Sci | © The Author [2013]. Published by Oxford University Press. All rights reserved. For Permissions, please

5 Figure 5. Optimization of the chiral separation of 6R-leucovorin on a Chirobiotic column with the following experimental conditions: 2 mM NH<sub>4</sub>TFA in MeOH–AcOH (100:0.2, v/v), flow rate of 1.0 mL/min and 20°C (A); 10 mM NH<sub>4</sub>TFA in MeOH–AcOH (100:0.2, v/v), flow rate of 1.0 mL/min and 20°C (B); 10 mM NH<sub>4</sub>TFA in MeOH–AcOH (100:0.2, v/v), flow rate of 1.2 mL/min and 40°C (C); MeOH–10 mM NH<sub>4</sub>TFA–AcOH (60:40:0.16, v/v), flow rate of 0.7 mL/min and 20°C (D) (34). From: Recent Advances in SPE–Chiral-HPLC Methods for Enantiomeric Separation of Chiral Drugs in Biological Samples J Chromatogr Sci. 2013;51(7): doi: /chromsci/bms262 J Chromatogr Sci | © The Author [2013]. Published by Oxford University Press. All rights reserved. For Permissions, please

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