1. Aim #43: How do scientists utilize recombinant DNA technology?
Date: January 23rd, 2017
Aim #43: How do scientists utilize recombinant DNA technology?
HW:
Quarterly Exam on Wednesday 1/25
Aim #’s 21-41
Unit 3 (Cells and Cell Transport) & State Lab- DTM
Unit 4 (Photosynthesis and Respiration)
Unit 5 (Genetics)
Quarter ends Friday! Please submit late labs no later than Friday 1/27!
Do Now:
What is the human genome project?
What are the steps?
Why is it so important?

2. Aim #43: How do scientists utilize recombinant DNA technology?
Why does Spiderman have
these special powers?

3. 1) What is Genetic Engineering?
Genetic Engineering is a new process that scientists
use to alter the genetic instructions in organisms.

4. Review: What is a Gene? A gene is a sequence of DNA on a chromosome
that codes for ________ protein.
one

5. Review: Why would altering DNA affect our characteristics/traits?
DNA codes for the proteins that determine our traits.

6. 2) Why would scientists want to alter the genetic instructions of organisms?+
Scientists can generate
organisms with desired
characteristics.

7. 3) How is DNA Modified? + Step 1: DNA is first extracted (removed)
from cells.
..ATGAATTCT..
..TACTTAAGA.. +
..ATGAATTCT..
..TACTTAAGA..
DNA
DNA

8. How is DNA Modified? Step 2: DNA is then cut into smaller pieces with
Restriction Enzymes.
AGTTATTC
TCAATAAG
A GTTATTC
TCAATAA G
Restriction enzymes are “scissor-like”
enzymes that recognize and cut
specific sequences in the DNA.
What base sequence was this
restriction enzyme specific for? GA

9. What base sequences were these Restriction Enzymes specific for?
“Sticky Ends”
ATGAATTCT
TACTTAAGA
ATG AATTCT
TACTTAA GA GA
“Sticky Ends”
GCATTACGTT
CGTAATGCAA
G CATTAC GTT
C GTAATG CAA GC

10. How is DNA Modified?
Step 3: The cut DNA sequence is then combined with
the DNA of another organism.
AATTCT AAGTACT
TACTTAA GATTCAT
AATTCTAAGTACT
TACTTAAGATTCAT +
DNA
from 2 sources
Cut DNA from
another cell
Cut DNA

11. What do we call DNA that comes from 2 sources?
Recombinant DNA

12. Gene Splicing enzyme The process that allows scientists to
attach pieces of DNA segments to the
DNA of other organisms is called:
GENE SPLICING

13. How is DNA Modified?
Step 4: The recombinant DNA is then placed inside a
living cell.
“Cut DNA” +
Recombinant DNA
What will the recombined DNA do?
It will allow for the production of
proteins from both sources of DNA.

14. 4) Applications of Genetic Engineering:
Scientists can target and
identify a gene.
Cut the gene with restriction
enzymes.

15. Applications of Genetic Engineering:
This gene is then “spliced”
to the DNA of another
organism.
The recombinant DNA is placed in the cells of another organism, and will produce proteins from both organisms.

16. Aim #43: How do scientists utilize recombinant DNA technology?
Date: January 24th, 2017
Aim #43: How do scientists utilize recombinant DNA technology?
HW:
Quarterly Exam on Wednesday 1/25
Aim #’s 21-41
Unit 3 (Cells and Cell Transport) & State Lab- DTM
Unit 4 (Photosynthesis and Respiration)
Unit 5 (Genetics)
Quarter ends Friday! Please submit late labs no later than Friday 1/27!
Do Now:
What are restriction enzymes and how do they work?
What is gene splicing?
What do you call DNA from two sources that has been spliced together?