1. Lab Safety National University JMARION@NU.EDU jclark@nu.edu
anatomy

2. National University Lab
Goals
Safe
Follow All Lab Rules
Wear Proper Clothes
Know What To Do In An Emergency
Clean
Put Materials Back Where You Took Them From
Wipe Down Work Stations And Clean Equipment Before And After Use
Use Trays To Prevent Major Spills
Fun

3. National University Lab
Who is Responsible for Making the Lab Clean, Safe, and Fun?
We all are!!

4. Responsibilities Instructors Demonstrates Proper Use of Equipment
Oversees Safety of Students While Class is in Lab
Makes Sure Student Areas Are Clean Before Dismissing Class

5. Responsibilities Students
Knows What the Lab Procedure is Before Starting the Experiment
Wears Proper Clothing For Lab Work (Will Be Discussed Later)
Disposes of Waste in Correct and Designated Areas
Pigs, Cats, Organs into Red Hazardous Waste Bins
Glass into Sharps Container
Personal Microscope Slides into Side Bin (Also Red)
Please do not dispose of Pre-Made slides
Cleans Supplies and Work Area After Use
Returns Cleaned Supplies Back to Designated Area

6. Responsibilities Lab Technicians
Prepares and Sets Up Equipment and Supplies Before Lab
Labels Supplies
Calibrates Equipment as Needed
Helps Instructor with Safety and Instruction
Lab Techs are Available for Questions Regarding Equipment Use, Calibration, and Trouble Shooting
Manages Used Supplies After Class and Checks Cleanliness of Lab
Reviews Lab Cleanliness and Procedure with Instructor After Class

7. Rule #1
Know the safety rules and procedures that apply to the work being done.
Review Potential Hazards and take appropriate safety precautions before beginning any new operations.
Practical Jokes or other behavior that might confuse, startle, or distract another lab student is not allowed.

8. Rule #2
Shower Head
Know where emergency equipment is located and how to properly use it.
Familiarize yourself with emergency routes.
In case of evacuation, SHUT OFF BUNSEN BURNERS BEFORE LEAVING.
MSDS Data
Shower Lever
First Aid Kit
Eye Wash Station
Spill Kit
Fire Blanket

9. Rule #3
Become Familiar with the types of protective equipment available and understand their use and limitations
Safety glasses are required whenever hazardous chemicals or equipment is being used, prepared, or demonstrated
Lab coats and Gloves, including disposables, are highly recommended.

10. Rule #4 No food or beverages are permitted in the Lab
Do not apply cosmetics in areas where chemicals are being used or stored

11. Rule #5
Confine long hair, jewelry, and loose clothing when working in the laboratory.
Appropriate shoes are required in the lab at all times.
Skin should be covered at all times.

12. Rule #6 Follow accepted waste disposal procedures
Work defensively with Flammables

13. Waste Disposal Glassware that has not touched Hazardous Waste
Hazardous Waste Slides
Culture Tubes and Plates
Glassware that has touched Hazardous Waste

14. Waste Disposal Cont… Biohazard Specimen Broken Glass Only
Autoclaved Materials

15. Back Packs and Briefcases

16. Proper Storage of Microscope
Eyepiece facing handle
Objective Lens set to 10x
Stage lowered and set back as far as possible
Cord loosely folded. Do not wrap the Cord around the base or neck of the Microscope.
Place Microscope in Cabinet with handle facing outwards

17. Lab Station Supplies 2 Lab Coats 2 Wire Loops 2 Wire Needles
1 Slide Container
1 Box of Slides
2 Clothes Pins
1 Ruler
1 Wax Pencil

18. Chemical Supplies 2 - 250 mL beakers 1 Bottle 95% Ethanol
1 Dropper Bottle Methylene Blue
1 Dropper Bottle Safranin
1 Dropper Bottle Crystal Violet
1 Dropper Bottle Iodine