1. Aim: How does temperature and pH affect enzyme activity?

2. LO: SWBAT describe factors that affect the rate of enzyme activity
DN: Explain the relationship between a substrate and an enzyme.
HW: Page 58 # 15, 16, 19, 20, 21, 22
Wednesday: Castle Learning (enzymes)

3. LO: SWBAT test for the presence of starch in different samples by using starch indicator.
DN: What do we use to test for the presence of starch? What will happen if starch is present?
HW: Page 58 # 15, 16, 19, 20, 21, 22
Wednesday: Castle Learning (enzymes)

4. What do we mean when we say reaction rate?
How can we speed up
reactions?
Add Enzymes
Another word for reaction rate, is the speed of a reaction.

5. A + B AB How do enzymes work?
Enzymes bind to the reactants so the chemical
reaction can take place.
ENZYME
A + B AB

6. How can we speed up a chemical reaction even more?
Increase enzyme concentration
Increase substrate concentration

7. How can we speed the number of cars that can be built?
Increase workers
Increase car parts
(Enzymes)
(Substrates)

8. Increasing substrate- concentration
As substrate
Concentration
increases,
The reaction rate
increases &
then remains
The same
Enzyme
A + B
AB + H2O

9. Increasing enzyme concentration
As enzyme
Concentration
increases,
The reaction rate
increases &
then remains
The same
Enzyme
A + B
A-B + H2O

10. How can we increase the rate of a chemical reaction?
How can we speed up
enzyme activity?

11. So, how do we speed up this chemical reaction?
Enzyme
A + B
AB + H2O

12. What is the relationship between Temperature & enzyme activity?
As T increases, enzyme activity increases up to T = ___ and then decreases

13. What is the optimum temperature for this enzyme?

14. What is the optimal Temperature for human enzymes?

15. What is the range at which this enzyme works?

16. Why does Enzyme activity begin to decrease after the optimum Temperature?
The enzyme changes shape!

17. So, how much have you learned?
What is the temperature range at which A, B, C work at?
At what range are A & B active at the same time?
What are their optimum temperatures?
At what temperature does B begin to denature? C A B

18. What is PH?

20. What is the relationship between pH and enzyme activity?
As pH increases from _______ to _________, enzyme activity increases and then decreases

21. When is enzyme activity at its greatest?

22. What is the optimal pH for human enzymes?

23. What is the range at which this enzyme works?

24. Why does Enzyme activity begin to decrease after the optimum pH?
DENATURE:
Loses shape

25. Pepsin & Trypsin
enzymes in our body that help proteins into amino acids.
hydrolyze
Pepsin
Trypsin

26. pH in stomach is about 2 Are the stomach juices acidic or basic?

27. So, how much have you learned?
What is the pH range at which A & B work at?
At what range are they both active?
What are their optimum pH’s?
Would enzymes A & B be found in the same location in our body? Why?

28. Why are Enzymes so pHaT?