1. Molecular Epigenetics
Biol 456/656
Molecular Epigenetics
Lecture #3
Aug 28, 2015

2. Videos explaining Illumina Hi-Seq
Reversible terminator chemistry

3. See Brown_Celniker_Nature_2014.pdf

4. Figure 1
90% of genes express at least 10 transcripts and 5 protein isoforms.
1% of genes have complex patterns of alternative splicing, promoter usage and polyadenylation, and can be processed into 100s of transcrtips.

5. Figure 1
What is CAGE?

6. Figure 1
Dystrophin 3’ end

7. Figure 1 Go to: chrX:4,892,228-4,981,117 ChrX:4850000-4950000
Go to: chrX:4,892,228-4,981,117

8. Figure 2

9. Figure 2
chrX:15,753,078-15,761,360

10. Figure 2
Per cent spliced in index

11. Figure 3

12. Figure 3

13. Figure 4

14. Figure 5

15. Figure 6

16. Homework: Read “Diversity and dynamics of the Drosophila transcriptome”
1. Draw or explain in words “gene models” illustrating:
A. First exon variant alternative splicing
B. Convergent transcription
C. Antisense transcription
2. Use the UCSC genome browser to find examples of the above events (1A, 1B, 1C)
and take screenshots of these. Use arrows and text boxes to point out relevant information.
3. Identify 3 aspects of the paper (a figure, technique, approach or statement made)
that you completely do not understand.
4. Explain, briefly, how you attempted to read the paper.
5. What would you score this paper out of 10?
me your answers prior to Monday’s class in a word, pages or powerpoint file.