1. BME 130 – Genomes Lecture 20 Gene expression and mRNA processing

2. Administrivia Homework 2 grading will be finished today Midterm 2 is Friday Final paper presentations are next Wednesday (November 17) Final or writing assignment – your choice

3. Figure 12.1 Genomes 3 (© Garland Science 2007) The simple chemistry of RNA polymerization

4. Figure 12.2 Genomes 3 (© Garland Science 2007) The RNA polymerase complex is held together in part through interactions between the template DNA and the RNA product at the transcription bubble

5. Figure 12.3 Genomes 3 (© Garland Science 2007) Extensive interactions between bacterial RNA polymerase, template, and nascent RNA product

6. Template DNA strand Non-template DNA strand RNA polymerase beta RNA polymerase beta’ RNA product

7. Figure 12.5 Genomes 3 (© Garland Science 2007) Intrinsic terminator

8. Figure 12.6 Genomes 3 (© Garland Science 2007)

9. Figure 12.7 Genomes 3 (© Garland Science 2007) Rho (helicase) dependent termination

10. Figure 12.10 Genomes 3 (© Garland Science 2007)

11. Figure 12.11 Genomes 3 (© Garland Science 2007) Attenuation – feedback operon control

12. Figure 12.16 Genomes 3 (© Garland Science 2007) rRNA expression One transcript Endonucleases separates each rRNA gene; Exonucleases trim these

13. Figure 12.17 Genomes 3 (© Garland Science 2007)

14. Figure 12.18 Genomes 3 (© Garland Science 2007) tRNAs and rRNAs are extensively modified

15. Figure 12.19 Genomes 3 (© Garland Science 2007) Bacterial RNA degradation

16. Eukaryotic pre-mRNA expression

17. Figure 12.20 Genomes 3 (© Garland Science 2007)

18. Figure 12.21 Genomes 3 (© Garland Science 2007) Eukaryotic RNAPol II transcripts have a 7-methyl-G cap

19. Figure 12.22 Genomes 3 (© Garland Science 2007) Eukaryotic 3’ end processing

20. Figure 12.25 Genomes 3 (© Garland Science 2007) Splicing

21. Table 12.2 Genomes 3 (© Garland Science 2007) Intron flavors

22. Table 12.3 Genomes 3 (© Garland Science 2007) Intron content varies widely

23. Figure 12.26 Genomes 3 (© Garland Science 2007)

24. Figure 12.27 Genomes 3 (© Garland Science 2007)

25. Figure 12.30 Genomes 3 (© Garland Science 2007)

26. Figure 12.31 Genomes 3 (© Garland Science 2007) SR proteins are trans-acting splicing factors

27. Figure 12.32a Genomes 3 (© Garland Science 2007)

28. Figure 12.32b Genomes 3 (© Garland Science 2007)

29. Figure 12.33a Genomes 3 (© Garland Science 2007)

30. Figure 12.33b Genomes 3 (© Garland Science 2007)

31. Figure 12.33c Genomes 3 (© Garland Science 2007)

32. Figure 12.35 Genomes 3 (© Garland Science 2007) Trans-splicing (C. elegans)

33. Figure 12.36 Genomes 3 (© Garland Science 2007)

34. Figure 12.42 Genomes 3 (© Garland Science 2007) RNA editing

35. Table 12.5 Genomes 3 (© Garland Science 2007)

36. Figure 12.44 Genomes 3 (© Garland Science 2007) Eukaryotic mRNA degradation

37. Figure 12.45a Genomes 3 (© Garland Science 2007)

38. Figure 12.45b Genomes 3 (© Garland Science 2007) Nonsense-meditaed mRNA decay

39. Alternative splicing and NMD to control gene expression

41. Figure 12.47 Genomes 3 (© Garland Science 2007) RNA interference (RNAi)

42. Figure 12.48 Genomes 3 (© Garland Science 2007)

43. Figure 12.49 Genomes 3 (© Garland Science 2007)