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Assessment of microvascular endothelial function in type 1 diabetes using laser speckle contrast imaging  Alessandra S. de M. Matheus, Eliete Leão Silva.

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Presentation on theme: "Assessment of microvascular endothelial function in type 1 diabetes using laser speckle contrast imaging  Alessandra S. de M. Matheus, Eliete Leão Silva."— Presentation transcript:

1 Assessment of microvascular endothelial function in type 1 diabetes using laser speckle contrast imaging  Alessandra S. de M. Matheus, Eliete Leão Silva Clemente, Maria de Lourdes Guimarães Rodrigues, Débora Cristina Torres Valença, Marília B. Gomes  Journal of Diabetes and Its Complications  Volume 31, Issue 4, Pages (April 2017) DOI: /j.jdiacomp Copyright © 2017 Elsevier Inc. Terms and Conditions

2 Fig. 1 Laser speckle contrast imaging studies skin microcirculation on the forearm. A, Laser head; B, Sphygmomanometer for occlusion; C, Electrode (−); D, Electrode (+) filled with Acetylcholine; E-Electrode control filled with NaCl; F, Vacuum cushion. Journal of Diabetes and Its Complications  , DOI: ( /j.jdiacomp ) Copyright © 2017 Elsevier Inc. Terms and Conditions

3 Fig. 2 Cutaneous vascular conductance with acetylcholine and post-occlusive reactive hyperemia. A comparison of peak microvascular responses to Acetylcholine (ACh) and post-occlusive reactive hyperemia (PORH) in healthy volunteers and patients. The amplitudes of vascular responses are expressed on cutaneous microvascular conductance (CVC, expressed in laser Speckle arbitrary perfusion units, LSPU, divided by mean arterial pressure in mmHg). The values are mean±SD. **p<0.001. Journal of Diabetes and Its Complications  , DOI: ( /j.jdiacomp ) Copyright © 2017 Elsevier Inc. Terms and Conditions

4 Fig. 3 Examples of skin microvascular flow obtained using laser speckle contrast imaging Legend: Graphic example of a typical exam performed using Laser speckle contrast imaging. A, HEALTHY control’s exam. Phase 1 means 3 minutes of registering resting flux before 6 increasing doses of Acetylcholine (ACh) iontophoresis. Phase 2 means Post occlusive reactive hyperemia (PORH) performed immediately after ACh iontophoresis. B, Type 1 Diabetes patient with endothelial dysfunction's exam. ROI: Region of interest Journal of Diabetes and Its Complications  , DOI: ( /j.jdiacomp ) Copyright © 2017 Elsevier Inc. Terms and Conditions

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