1. Hand Hygiene Indicator
Emily Andrews
Allie Finney
Rachel Mosher
Susie Samreth
RACHEL

2. Presentation Outline Motivation Existing Products Problem Statement
Client Goals
Experimental Design
Experimental Results
Fluorescent Molecules
Future Work
RACHEL

3. Motivation Prevent transmission of healthcare-associated infections
20,000 deaths per year
Importance of hand hygiene
Hospital Video Auditing
Skin Surface pH Testing
Flourescent Powder
EMILY
-Hospital deaths due to communicable diseases contracted in hospital
-Hand hygiene training for hospital staff
-Alternatives
-Focus on use of commercial products that utilize fluorescence
According to the U.S. Centers for
Disease Control and Prevention (CDC),
one-third of all HAIs can be prevented
through the implementation of a
hand hygiene program — but hand
hygiene compliance in healthcare
facilities is reported to average only
20 to 50 percent.

4. Existing Products Commerical Products Applications GloGerm Glitterbug
Proper hand washing techniques
Demonstrate transmission
in mock environment
EMILY
-GloGerm demonstration
-Emphasize that GloGerm simulates bacteria, does not actually attach to bacteria
-Increased use of alcohol-based hand sanitizer
-Need for GloGerm analog compatible with alcohol-based hand sanitizer

5. Problem Statement Increased use of hand sanitizer
Existing products not effective for training
Similar product compatible with hand sanitizer
EMILY
-Increased use of hand sanitizer instead of traditional soap and water hand washing
-Existing products may not be effective in attenuating fluorescence
-Need for GloGerm analog compatible with alcohol-based hand sanitizer

6. Client Goals Topical Agent for hand hygiene training
Fluorescent molecule to simulate bacteria
Attenuation after hand sanitization
Qualitative change
UV light wavelength
SUSIE
-Overarching goal of preventing transmission of hospital-related infections through improved hand hygiene training
-Intrinsic fluorescence of molecule safe for application to skin
-Fluorescence should be attenuated with application of alcohol-based hand sanitizer
-Trainees should observe visible change in fluorescence to indicate hand cleanliness
-Eventually tag bacteria with topical agent for real-time auditing of staff hand hygiene…this requires spectrofluoremeter
-Spectrofluorometers: large, permanent equipment
-Goal: portable, hand-held, real-time measurement with quantitative data
-For staff surveillance and research on hand hygiene and transmission using quantitative data
-This semester will focus on topical agent that simulates bacteria!!!

7. Product Requirements Change in fluorescence with EtOH FDA approval
Skin application safety
Existing patent
Commercial products
Solubility in H20
Cost

8. Experimental Design SUSIE
-obtain sample  cuvette  spectral collection  OH addition  spectral collection  data analysis
Note assumptions:
-Cuvette simulates hand
-Ethanol utilized in OH addition instead of ethanol-based hand sanitizer
-dissolution of some samples difficult
Currently experimenting at excitation of 350 nm…specific ranges to be determined following additional testing…targeting UV light wavelength ranges because of clinical relevance of training

9. Experiment Results
ALLIE

10. Experiment Results
ALLIE

11. Fluorescent Molecules
Product
Change in Fluorescence with EtOH
FDA approval
Existing Patent
Solubility in H20
(1-5)
Cost
(per ounce)
GloGerm
69.67% No
Yes 3
$2.10
Glitterbug
3.85% 2
$8.30
Tyrosine
43.56% 4
$9.40
Tryptophan
1.18%
Phenylalanine
27.15% 1
$15
RACHEL
Not like typical design matrix because research based

12. Future Work Brainstorm and research Quantify qualitative data
Coumarin
Chlorophyll
Vitamin B12
Fluorescein
Quantify qualitative data
Spectrofluorometry
Research fiber optics
Continue testing
Optimize alcohol quantity
Optimize spectrofluorometer settings
Immitate skin application
RACHEL

13. Questions?

14. Acknowledgements Professor Bill Murphy Dr. Chris Crnich Kevin Elicieri
Professor Jim Weisshaar

15. References
Report/ProductsServicesProtectHealth.pdf
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oject/images/emission_spectrum.gif