1. Measurement of Copper Nanoparticle Concentration Using Surface Plasmon Resonance
Amir Reza Sadrolhosseini
Dr. A.S.M. Noor, Professor Dr. M. A. Mahdi
Excellence of Wireless and Photonics Networks Research Center (WiPNET ),
Universiti Putra Malaysia,
43400 Serdang, Selangor, Malaysia

2. Outline Basic of SPR SPR setup Preparation of Sensing Layer based on polymer material Preparation of Cu-NPs Presentation of SPR Sensor

3. Surface plasmon Resonance (SPR)
Amir Reza Sadrolhosseini GS17153
Surface plasmon Resonance (SPR)
The SPR is an optical –electrical phenomenon .
This phenomenon due to charge density oscillation at the interface of two media with dielectric constants of opposite sign.
Application of SPR :
Characterizing the new material.
( )
Sensor
Microscopy and Spectroscopy
Coupling Methods :
Prism Coupling
Grating Coupling
Waveguide
n=1.83
Modulation Methods
Wavelength Modulation
Phase Modulation
Intensity Modulation
Polarization Modulation
Angular Modulation
n= i
er =-10.24
ei=1.605
Thickness=52.5 nm
He-Ne Laser
632.8nm

4. Surface Plasmon Resonance setup Setup
SPR setup
He-Ne laser
Chopper
Polarizer
Pinhole
Photo detector
Precision rotation stage
Lock in Amplifier
Computer

5. Procedure of this project
Preparation of Polypyrrole Multi-walled Carbon Nanotube(PPy-MWCNTs)
layer.
Characterization of layer.
Measurement of layer thickness.
Preparation of copper nanoparticle (Cu-NPs) in virgin oil.
Characterization of Cu-NPs.
Measurement of Cu-NPs concentration Using SPR sensor.

6. Preparation of Polypyrrole Multi-walled Carbon Nanotude layer (PPy-MWCNTs)
1- The gold layer was prepared with a sputtering coating on a glass slide at a thickness of 49 nm.
2- The PPy-MWCNTs layer were synthesized by electrochemical polymerization of distillated pyrrole on MWCNT
Dissolving the sodium dodecylbenzensulfonate (SDBS) in distilled water
The ratio of nanotubes to SDBS was 1:10.
The ratio of MWCNTs to Py was 0.7%
The suspension sonicated for four hours to enhance the disaggregation of any
nanotube bundles.
The PPy-MWCNT premixed solution was electropolymerized at +0.7 V for different time on gold- coated glass slide at room temperature.

7. Characterization of Layer
Sample
Angle of
Resonance
Thickness
(nm)
Reflectance
Refractive index
20 s
54.41
9.2
0.112 i
31 s
55.139
12.6
0.152 i
41 s
56.971
21.1
0.244 i
51 s
58.319
28.3
0.311 i
60 s
59.618
36.4
0.376 i

8. Preparation of Copper Nanoparticle in Virgin Coconut Oil Using Laser Ablation
Setup
UV-Visible Spectrum
FT-IR Result
Q switch Nd:YAG laser : 532 nm,
Energy: 1200 mJ, 10 ns,
Repetition time : 40 Hz

9. Morphology and particle size
Size:11nm
N: 57
Size:10 nm
N:102
Size: 7 nm
N:180
Size: 4 nm
Particle Size : 11nm nm

10. Measurement of concentration of Cu-NPs
Prism/Gold/PPy-MWCNTs/DDW
Prism/Gold/PPy-MWCNTs/virgin coconut oil
In virgin oil
In water

11. Amir Reza Sadrolhosseini GS17153
Kinetic Behavior
Association Process association constant
A+B=>AB
Dissociation Process dissociation constant
AB =>A+B
Response of sensor ε~M×γ γ is surface concentration of form complexes
Maximum Response ξ~M×β M is mass of analyte
Normalized value ε /ξ= γ/ β β is the surface concentration of receptor
Critical Distance
Total Complex concentration

12. Maximum value of resonance angle shift
Analysis of data based on kinetic theory
Sensogram
=sat(1-exp(-kat)) ,
=maxCK/(1+KC)
Langmuire’s first order adsorption model
K=1.781
max=0.4026
Maximum value of resonance angle shift
Affinity constant
AAS: 0.069, 0.11, 0.34 and 0.83 mg/L
SPR: 0.072, 0.09, 0.35 and 0.82 mg/L

13. Advantages SPR Method NO need to know chemical knowledge
SPR sensor is cheapper than other methods such as
Atomic Absorption Spectroscopy (AAS)
Inductively coupled plasma-mass spectrometry (ICP-MS)
Portability
No calibration for each experiment.
Laser Ablation
Setup

14. Laser ablation is green method for preparation of Cu-NPs in oil
Conclusion
Laser ablation is green method for preparation of Cu-NPs in oil
The particle size shifted from 11 to 4 nm with increasing the ablation time.
The concentration of Cu-NPs was measured successfully using PPy-MWCNTs composite layer with sensitivity about 0.01 ppm.

15. Thank you so much to Pay Attention me