1. Microprobe activities in Lund
An overview

2. Overview University of Lund/ LTH Physics – applied Nuclear Physics
Micro beam, PIXE and singe cell facility
Additional /potential resources
The irradiation facility, Status, plans and future.
Human resources, PhD program

3. Lund University (LU) (http://www. lu
Lund University (LU) ( ) is a research intensive and comprehensive university with eight faculties,
about students, (City of Lund )
6500 employees,
and 3000 PhD students (460 PhD exams/year)
The turnover is about 5000 million SEK

4. The Physics Department ( http://fysik.lu.se/ )
300 researchers and employees:
Experimental High Energy Physics
Nuclear Physics,
Synchrotron Radiation Physics,
Atomic Physics and
Solid State Physics

5. The Nuclear Microprobe group (http://micro. pixe. lth
The Nuclear Microprobe group ( is a main research group within the Division of Nuclear Physics
( and has a long and world class tradition in the field of Ion beam analysis using MeV ions for quantitative multi-element characterisation of microscopic structures, e.g. PIXE.
A second field of interest is the controlled use of single MeV ions to specifically create localised damage for special purposes; localised low dose irradiation of single living cells and direct write µm-scale proton lithography.

6. The irradiation facility is based on a single-ended 3 MeV electrostatic accelerator, high quality focusing lenses and similar equipment.
A facility for studying extreme low-dose irradiation of living cells is under development at the laboratory.
It is based on the technique to extract single MeV ions from the accelerator system, allowing studies on a cellular level of phenomena like mutagenic effects, bystander effect, adaptive response.
The group has a close cooperation with the Department of Radiation Physics in Lund regarding cell biology, and is also a part of the Lund University network Alliance for Biomedical Imaging and Bioengineering with competence in mathematical modelling and advanced image analysis.

7. The sub-micron line
Several years ago funding was given to construct the sub-micron beam line.
The Single cell irradiation activity started with the CELLION project.
Instrumentation was decided to be integrated in the coming sub-micron beam line that was already under construction: split quadrupole lens configuration, a new flexible irradiation chamber, new high vacuum beam lines, etc.

8. The Lund sub-micron beamline (not to scale)
Irradiation chamber
Beamviewer+ FC
HV-deflector
”Electrostatic chopper”
Cross-over
Aperture 2
QP-lens 1
Aperture 1
QP-lens 2

9. The irradiation chamber is built around a framework with flexible sides.
High vacuum pump

10. The irradiation chamber is built around a framework with flexible sides.
High vacuum pump

11. The irradiation chamber is built around a framework with flexible sides.
High vacuum pump

12. The irradiation chamber is built around a framework with flexible sides.
X_Y_Z
Obj.& det.
High vacuum pump
Backplane

13. The irradiation chamber is built around a framework with flexible sides.
X_Y_Z
Micro-scope and TV
Obj.& det.
Sample holder
High vacuum pump
Backplane

14. The irradiation chamber is built around a framework with flexible sides.
X_Y_Z
Micro-scope and TV
High vacuum pump

15. The irradiation chamber is built around a framework with flexible sides.
X_Y_Z
Micro-scope and TV
Beam Scanning coils
High vacuum pump

16. The irradiation chamber is built around a framework with flexible sides.
X_Y_Z
Micro-scope and TV
High vacuum pump

17. The irradiation chamber is built around a framework with flexible sides.
X_Y_Z
Micro-scope and TV
High vacuum pump

18. The irradiation chamber is built around a framework with flexible sides.
X_Y_Z
Micro-scope and TV
High vacuum pump

19. Single cell irradiation project
Existing facilities
CELLION
Funding for equipment (30 k€,Crafoord)
For the project, 80% goes into instrumentation development -including cell design, 20 % into biological programme.
Focused, horizontal beam
200 nm Si3N4 exit window
Particle detection after the cells (PIN diode)
Investigating the possibilities to use thín DE detectors as pre-cell detector

20. Swedish PhD studies/ CELLION E.S.R.
Courses (50 credits = 75 CTS)
Research training
Publications = 4 years (+ 20% teaching) = 5 years
Research training
Publications = 3 years year paid by Lund University => PhD

21. Web based protocol for PhD studies
Formalities (supervisor, subject, resources,..)
Year by year plan
Courses
Conferences
Research training
Publications

22. Micro beam group Natalia Arteaga (CELLION), PhD
Vaida Auzelyte (proton litography), PhD
Mikael Elfman (res. Eng.)
Per Kristianssson (prof)
Klas Malmqvist (prof)
Charlotta Nilsson, (master thesis done, CELLION,PhD in Leipzig)
Christer Nilsson (res. techn)
Jan Pallon ( CELLION) (Ass. Prof.)
Marie Wegdén (BNCT, H_H scattering, tomography), PhD
Fredrik Andersson (master thesis done) Bordeaux
Anna Mölder, master thesis