1. In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts 
Carla Renata Sipert, DDS, PhD, Ana Carolina Morandini, DDS, PhD, Thiago José Dionísio, MSc, Maria Aparecida Andrade Moreira Machado, DDS, PhD, Sandra Helena Penha Oliveira, PhD, Ana Paula Campanelli, PhD, Winston Patrick Kuo, DDS, DMSc, Carlos Ferreira Santos, DDS, PhD 
Journal of Endodontics 
Volume 40, Issue 1, Pages (January 2014)
DOI: /j.joen
Copyright © 2014 American Association of Endodontists Terms and Conditions

2. Figure 1
Characterization of cultured fibroblasts by FSP-1. Cultured GF (A), PLF (B), PDPF (C), and DDPF (D) were immunostained for FSP-1. Images captured by a confocal microscope (representative bars, 20 μm).
Journal of Endodontics  , DOI: ( /j.joen )
Copyright © 2014 American Association of Endodontists Terms and Conditions

3. Figure 2
Production of CCL3 (MIP-1α) by cultured fibroblasts. Cultured GF, PLF, PDPF, and DDPF were stimulated with EcLPS (A–C) and EfLTA (D–F) at the indicated concentrations. Cell supernatants were collected after 1 hour (A and D), 6 hours (B and E), and 24 hours (C and F). Production of CCL3 was detected by ELISA. *P < .05, **P < .01, and ***P < .001 in comparison with culture medium alone (0). Significant differences with #GF, with %PLF, and with &PDPF (n = 6).
Journal of Endodontics  , DOI: ( /j.joen )
Copyright © 2014 American Association of Endodontists Terms and Conditions

4. Figure 3
Production of CXCL12 (SDF-1) and expression of miR-141 and miR-200a by cultured fibroblasts. Cultured GF, PLF, PDPF, and DDPF were stimulated with EcLPS (A–C) and EfLTA (D–F) at the indicated concentrations. Cell supernatants were collected after 1 hour (A and D), 6 hours (B and E), and 24 hours (C and F). Production of CXCL12 was detected by ELISA. *P < .05, **P < .01, and ***P < .001 in comparison with culture medium alone (0). Significant differences with #GF, with %PLF, and with &PDPF (n = 6). Expression of miR-141 (G) and miR-200a (H) was detected by means of qPCR for EcLPS-stimulated cells for 24 hours (n = 6).
Journal of Endodontics  , DOI: ( /j.joen )
Copyright © 2014 American Association of Endodontists Terms and Conditions