1. SHRUTI PANDEY, SURESH PUROHIT, BRAHMESHWAR MISHRA, ANAND K. CHAUDHARY
DESIGN AND FORMULATION OF TRANSDERMAL DELIVERY SYSTEM FOR RASNA SAPTAK KWATH
SHRUTI PANDEY, SURESH PUROHIT, BRAHMESHWAR MISHRA, ANAND K. CHAUDHARY
DEPARTMENT OF RASA SHASTRA, FACULTY OF AYURVEDA, INSTITUTION OF MEDICAL SCIENCES,
BANARAS HINDU UNIVERSITY, VARANASI, UTTAR PRADESH
ABSTRACT
DISCUSSION
Table 01: Herbs present in Formulation
Sr. No
Plant Name
Scientific Name
Part Used 1
Rasna
Pluchea lanceolata
Leaf 2
Gokshura
Tribulus terristris
Fruit 3
Guduchi
Tinospora cardifolia
Stem 4
Punarnava
Boerihhia diffusa
Root 5
Eranda
Ricinus diffusa 6
Devdaru
Cedrus deodara 7
Aragvadha
Cassia fistula 8
Sunthi
Zingiber officinalis
Rhizome (dry)
Ayurveda (Indian System of Medicine) have a number of formulations for treatment of different diseases. Advancement in dosage form in Ayurvedic system of medicine is the demand of time. Rasna Saptak Kwath (RSK) is an oral dosage ayurvedic formulation which is given to the patient in decoction form to treat arthritis and to relieve inflammation and pain related to disease. This study was carried out with the aim of developing transdermal formulations viz. gel, spray or transdermal patch containing ingredients of RSK for the treatment of arthritis. RSK is a formulation of eight herbal drugs viz. Pluchea lanceolata,, Tribulus terristris, Tinospora cardifolia, Boerrihia diffusa, Ricinus communis, Cedrus deodara, Cassia fistula and Zingiber officinalis. Extractions of these herbs were carried out by two methods. The first one was carried out in hydro-alcoholic (50:50) media while the another was done through classical method of Ayurveda i.e. decoction method. The obtained extracts were dried in the oven which were tested for the presence of different phytochemical. In vitro drug releases of these extracts were also carried out through franz diffusion cell by using rat skin. Gallic acid was taken as a standard component. From the result, it was concluded that the aqueous extraction results in higher yield as well as phytoconstituent as compare to hydro-acloholic extraction. Result in vitro drug release showed more concentration of gallic acid in receptor media in which aqueous extract was used. Further investigations for these extracts and in vivo studies will be carried out to develop topical or transdermal dosage form.
The yield of two extracts i.e. hydro-alcoholic and aqueous extract of Rasna Saptak Kwatha were found to be 7% and 12% respectively.
Difference in yield in number of phyto-constituents present in these extracts.
Among them Gallic acid was taken as standard for in vitro drug release studies.
In permeation studies a aqueous extract showed more concentration of Gallic acid in receptor media when analyze through UV spectrophotometer.
After study the color of both receptor media were found to be changed i.e. from transparent to light brown color. and color of permeated extract might be the reason for this.
Table 02: Phyto-constituent Evaluation in Extracts
Sr.No
Phyto-constituent
Aqueous extract
Hydro-alcoholic extract 1
Alkaloid + - 2
Flavonoid 3
Phenol ++ 4
Tannin 5
Saponin 6
Terpenoid 7
Quinone
CONCLUSION
The result showed that aqueous extraction have higher percentage yield as well as more number of phyto-constituent present in it, in comparison to hydro-alcoholic extract
As the Gallic acid is permeating through the skin so further research and in vivo studies will help us to develop topical or transdermal dosage form from herbs of Rasna Saptak Kwatha formulation.
INTRODUCTION
REFERENCES
Arthritis is characterized by inflammation of the flexible joints leading to swelling, stiffness, restriction of movement of joints which can eventually result in cartilage damage. Rasna Saptak Kwath (RSK) is an Ayurvedic medicine which is frequently used in such condition and show effective therapeutics.
Because of 24 X 7 of busy schedule of society patients failed to followed the proper line of treatment. Patients need READY to USE treatment for their disorders.
So, in this research study we will formulate Transdermal Patch for controlled drug delivery system and spray for topical application from the herbs of RSK medicine.
OBJECTIVES:
1. To carry out extraction from the herbs of Rasna Saptak Kwath in different solvent media.
2. Evaluation for the different phyto-constituent present in these extracts.
3. In vitro drug release of the extracts using rat skin and Gallic acid was taken as standard for drug release study.
1. Anonymus, The Ayurvedic Formulary of India, Department of AYUSH, MH&FW, GOI, New Delhi, 2003, Part - I
2. Rohit kumar Bargah, Preliminary test of phytochemical screening of crude ethanolic and aqueous extract of Moringa pterygosperma. Gaertn Journal of Pharmacognosy and Phytochemistry 2015; 4(1): 07-09
3. Jirapornchai Suksaeree, et. Al. Zingiber cassumunar blended patches for skin application: Formulation, physicochemical properties, and in vitro studies. Asian Journal of pharmaceutical sciences 10 (2015)
Hydro-alcoholic Extraction through Soxhelet
Final Hydro-alcoholic E xtract(Hy-alc ext)
ACKNOWLEDGMENT
I am very much thankful to Ms. Pooja Mittal Ph.D Scholar, Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University) and Mr. Harsh Pandey, Ph.D. Scholar, Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu university for their helping hand while performing my experimental studies.
Extraction through Decoction Method
Final Aqueous Extract(Aq-ext.)
METHODS
The raw drugs were procured from the reliable sources of Varanasi. Extractions of these herbs were carried by two methods. One extraction was done in hydro-alcoholic (50:50) media through soxhelet process and solvent was recovered through rota evaporator while the another was done through classical method of Ayurveda i.e. decoction was made. Dried extracts were obtained .These two extracts were tested for the presence of different phytochemical constituent viz tannin, glycosides etc. In vitro drug release of these extracts were carried out through franz diffusion cell by using rat skin. 25 ml of Phosphate buffer was taken in receptor chamber and the temperature of receptor media was maintained at 37±20 C. Gallic acid was taken as a standard components. UV spectrophotometer was used for detection of gallic acid in media at 273 nm.
In Vitro Diffussion Study
Receptor Media-Aq . ext Receptor Media-Hy-Alc. ext
Standard Gallic Acid (GA)
GA in receptor media (Aq. ext)
GA in receptor media (Hy-alc. ext)
(Presented at 68th Indian Pharmaceutical Congress held at Vishakhapatnam during December 2016.)