1. Optikai mikroszkópok I.
Konjugált síkok a modern mikroszkópokban. Inverz, fáziskontraszt, epifluoreszcens mikroszkópok. Fluoreszcens fehérjék és optogenetika.
Irodalom:

2. Modern mikroszkópok felépítése, végtelenre korrigált optika

3. Konjugált síkok

4. Fáziskontraszt mikroszkóp

5. Fáziskontraszt mikroszkóp

6. Epifluoreszcens mikroszkóp

7. Fluoreszcens fehérjék

8. A GFP szerkezete

9. EYFP vektor
The basic plasmid vector configuration useful in fluorescent protein gene transfer experiments has several requisite components. The plasmid must contain prokaryotic nucleotide sequences coding for a bacterial replication origin for DNA and an antibiotic resistance gene. These elements, often termed shuttle sequences, allow propagation and selection of the plasmid within a bacterial host to generate sufficient quantities of the vector for mammalian transfections. In addition, the plasmid must contain one or more eukaryotic genetic elements that control the initiation of messenger RNA transcription, a mammalian polyadenylation signal, an intron (optional), and a gene for co-selection in mammalian cells. Transcription elements are necessary for the mammalian host to express the gene fusion product of interest, and the selection gene is usually an antibiotic that bestows resistance to cells containing the plasmid. These general features vary according to plasmid design, and many vectors have a wide spectrum of additional components suited for particular applications.

10. Brainbow

11. Optogenetika
Demonstration of single-component optogenetic control of neurons with microbial opsins4 was followed by corresponding optogenetic terminology2 in October 2006, and corresponding optogenetic control of freely moving mammals using microbial opsins and the fiberoptic neural interface9, 10. Also marked are identifications of bacteriorhodopsin3, halorhodopsin5 and channelrhodopsin6, all of which were much later (2005–2010) shown to function as fast, single-component optogenetic tools in neurons. Numbers indicate only publications searchable by 'optogenetics' or derivatives thereof on 1 December 2010.

12. Optogenetika