1. supplementary Fig. 1 Anti-HSP70 Ab Anti-SHP2 Ab
Anti-SHP2 Ab (Input)
IP : HSP70 Ab
IP : FLAG (SHP2) Ab
Anti-SHP2 Ab
Anti-HSP70 Ab
Anti-HSP 70 Ab (Input)
supplementary Fig. 1
FLAG SHP2 D.M.(EGF)
FLAG SHP2 D.M
FLAG SHP2 W.T.(EGF)
FLAG SHP2 W.T.
con
Sup Fig 1. Lysates from cells that were transfected transiently with the FSHP2WT or the FSHP2DM constructs
with or without EGF (100ng/ml) treatment for 10 min.
The cell lysates were immunoprecipitated with either FLAG or HSP70 antibodies,
followed by western blot analysis using SHP2 or HSP70 antibodies.

2. supplementary Fig. 2 Anti-4G10 Ab Anti-HSP70 Ab Anti-SHP2 Ab EGF 5 15
SHP2 DM/HSP70
SHP2 WT/HSP70
(cell lysates)
EGF 5 15
(min)
(kDa)
175 83 62 48 33 25
(100ng/ml)
supplementary Fig. 2
Sup Fig 2. Western blot analysis was carried out with lysates of the cells
transfected with SHP2WT or SHP2DM with HSP70 constructs.
Indicated antibodies were used to detect specific proteins.

3. supplementary Fig. 3A supplementary Fig. 3B FLAG(GAB1) HSP70 SHP2 GAB1+ –
SHP2WT
FLAGGAB1
Transfection
EGF (10 min)
Input
(100ng/ml)
supplementary Fig. 3A + –
HSP70
SHP2WT
FLAGGAB1
Transfection
EGF (10 min)
GAB1
SHP2
IP : SHP2 Ab (first lane with rabbit IgG)
IP : FLAG Ab (first lane with mouse IgG)
Phos-GAB1
PI-3 kinase
(100ng/ml)
supplementary Fig. 3B
Sup Fig 3A. Lysates were obtained from cells that had been transfected transiently
with the FGAB1, the SHP2WT/FGAB1 or the SHP2WT/FGAB1/HSP70 constructs.
The cell lysates were western-blotted with FLAG, HSP70, or SHP2 antibodies.
Sup Fig 3B. The binding level of SHP2 with GAB1, HSP70 or other tyrosine phosphorylated protein was
assessed by IP with SHP2 and FLAG antibodies. After IP, the indicated antibodies were used for western blot.