1. Title: Identification of loci associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis
Jounal: Plant Cell Report
Authours:
Hiroyasu Kitashiba1, Kumiko Taguchi1, Ikuyo Kaneko1, Kiyofumi Inaba1, Shuji Yokoi2, 3, Yoshihito Takahata2, and Takeshi Nishio1.
Affiliations and addresses of the authors:
1. Graduate School of Agricultural Science, Tohoku University
2. Faculty of Agriculture, Iwate University
3. Present address
Graduate School of Life and Environmental Science, Osaka prefecture University
Corresponding author:
Hiroyasu Kitashiba
Graduate school of Agricultural Science, Tohoku University
1-1, Tsutsumidori-Amamiyamachi, Sendai, Miyagi, , Japan
Tel:
Fax:

2. a
Bra (Bnm4D-84; 2,765 bp)
HM-DH1: CTA (Leu274)
CRS1 : GTA (Val274)
zf-Ring finger domain
( ) 5’
2765bp 3’
SNP1. HM-DH1: AGT (Ser298)
CRS1: AAT (Asn298)
SNP2. HM-DH1: GAG (Glu300)
CRS1: GAC (Asp300)
SNP3. HM-DH1: CTT (Leu301)
CRS1: CAT (His301)
100bp b
Bra (CLE-like; 288 bp)
Signal Peptide 5’ 3’
SNP1. HM-DH1: GTT (Val19)
CRS1: TTT (Phe19)
SNP2. HM-DH1: GTA (Val20)
CRS1: GCA (Ala20)
50bp c
Bra (HSP70B; 1,953 bp)
Three conserved regions
Peptide binding domain 5’ 3’
100bp
HM-DH1: AGG (Arg251)
CRS1: AAG (Lys251)
Supplementary Fig. 1. Differences of amino-acids by non-synonymous substitution for each gene between ‘HM-DH1’ allele and ‘CRS1’ allele.