Presentation is loading. Please wait.

Presentation is loading. Please wait.

Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,

Published byDonald Cole Modified over 6 years ago

Similar presentations

Presentation on theme: "Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,"— Presentation transcript:

1 Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression 
Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas, PhD  Journal of Allergy and Clinical Immunology  Volume 112, Issue 3, Pages (September 2003) DOI: /S (03)

2 FIG 1 In vitro response in cultured splenocytes. A, No difference between control (□) and vitamin D–treated (■) mice after unspecific stimulation was seen. However, there was an increase in OVA-specific response in the vitamin D–treated group. B, Proliferation was suppressed by means of in vitro addition of vitamin D in both vitamin D–treated (■) and control (□) mice. C, OVA-specific cytokine production by spleen cells in vitamin D–treated (■) and control (□) mice is shown. IL-5 levels were significantly decreased in vitamin D–treated mice. Histograms show values expressed as means ± SEM (n = 6 mice per group). ∗P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

3 FIG 2 Serum levels of OVA-specific IgE (A) and IgG1 (B) in control (□) and vitamin D–treated (■) mice determined by means of ELISA. Histograms show values expressed as means ± SEM in picograms per milliliter. ∗P < .05; ∗∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

4 FIG 3 Cytokine levels in BAL fluid determined by means of ELISA in control (□) and vitamin D–treated (■) mice. IFN-γ levels were undetectable. Histograms show values expressed as means ± SEM in picograms per milliliter. ∗P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

5 FIG 4 A, Eosinophils in BAL fluid and lung samples were decreased in vitamin D–treated (■) compared with control (□) mice. B, Eosinophils, with their characteristic brown color, are observed in a collapsed airway with a mucus plug from a control mouse. C, Few eosinophils are seen in a vitamin D–treated mouse. D, Percentages and total numbers of eosinophils in IL-4−/− mice were decreased in vitamin D–treated mice. The number of affected airways with a presence of airway epithelial mucus cells was also diminished after treatment with vitamin D. Percentages and total numbers of eosinophils in airways of IL-4−/− mice were decreased in vitamin D–treated mice. Histogram shows values expressed as means ± SEM. ∗P < .05; ∗∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

6 FIG 4 A, Eosinophils in BAL fluid and lung samples were decreased in vitamin D–treated (■) compared with control (□) mice. B, Eosinophils, with their characteristic brown color, are observed in a collapsed airway with a mucus plug from a control mouse. C, Few eosinophils are seen in a vitamin D–treated mouse. D, Percentages and total numbers of eosinophils in IL-4−/− mice were decreased in vitamin D–treated mice. The number of affected airways with a presence of airway epithelial mucus cells was also diminished after treatment with vitamin D. Percentages and total numbers of eosinophils in airways of IL-4−/− mice were decreased in vitamin D–treated mice. Histogram shows values expressed as means ± SEM. ∗P < .05; ∗∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

7 FIG 4 A, Eosinophils in BAL fluid and lung samples were decreased in vitamin D–treated (■) compared with control (□) mice. B, Eosinophils, with their characteristic brown color, are observed in a collapsed airway with a mucus plug from a control mouse. C, Few eosinophils are seen in a vitamin D–treated mouse. D, Percentages and total numbers of eosinophils in IL-4−/− mice were decreased in vitamin D–treated mice. The number of affected airways with a presence of airway epithelial mucus cells was also diminished after treatment with vitamin D. Percentages and total numbers of eosinophils in airways of IL-4−/− mice were decreased in vitamin D–treated mice. Histogram shows values expressed as means ± SEM. ∗P < .05; ∗∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

8 FIG 4 A, Eosinophils in BAL fluid and lung samples were decreased in vitamin D–treated (■) compared with control (□) mice. B, Eosinophils, with their characteristic brown color, are observed in a collapsed airway with a mucus plug from a control mouse. C, Few eosinophils are seen in a vitamin D–treated mouse. D, Percentages and total numbers of eosinophils in IL-4−/− mice were decreased in vitamin D–treated mice. The number of affected airways with a presence of airway epithelial mucus cells was also diminished after treatment with vitamin D. Percentages and total numbers of eosinophils in airways of IL-4−/− mice were decreased in vitamin D–treated mice. Histogram shows values expressed as means ± SEM. ∗P < .05; ∗∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

9 FIG 5 Time table for vitamin D's effects. A, IgE levels are increased in all groups treated with vitamin D. B, IgG1 is decreased in all groups receiving early vitamin D treatment at sensitization from day −1 to day 3. C, Vitamin D downregulates eosinophilia as a late-stage effect when given at challenge from day 5 to day 9. ∗P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

Download ppt "Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,"

Similar presentations

Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein  Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.

Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein  Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.
Heat-killed Lactobacillus plantarum L-137 suppresses naturally fed antigen–specific IgE production by stimulation of IL-12 production in mice  Shinji.

Heat-killed Lactobacillus plantarum L-137 suppresses naturally fed antigen–specific IgE production by stimulation of IL-12 production in mice  Shinji.
IL-4 induces IL-13–independent allergic airway inflammation

IL-4 induces IL-13–independent allergic airway inflammation
Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M

Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M
Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme  Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,

Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme  Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,
CD4 T-helper cells engineered to produce IL-10 prevent allergen-induced airway hyperreactivity and inflammation  Jae-Won Oh, MD, PhD, Christine M. Seroogy,

CD4 T-helper cells engineered to produce IL-10 prevent allergen-induced airway hyperreactivity and inflammation  Jae-Won Oh, MD, PhD, Christine M. Seroogy,
Prior airway exposure to allergen increases virus-induced airway hyperresponsiveness  Mika J Mäkelä, MD, Ralph Tripp, PhD, Azzeddine Dakhama, PhD, Jun-Won.

Prior airway exposure to allergen increases virus-induced airway hyperresponsiveness  Mika J Mäkelä, MD, Ralph Tripp, PhD, Azzeddine Dakhama, PhD, Jun-Won.
Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,

Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,
Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.
Local administration of antisense phosphorothioate oligonucleotides to the c-kit ligand, stem cell factor, suppresses airway inflammation and IL-4 production.

Local administration of antisense phosphorothioate oligonucleotides to the c-kit ligand, stem cell factor, suppresses airway inflammation and IL-4 production.
Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,

Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,
Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,

Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,
Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.

Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells  Shigeru Okumura, DDS, PhD, Hironori Sagara,

FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells  Shigeru Okumura, DDS, PhD, Hironori Sagara,
Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,

Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,
Role of IL-9 in the pathophysiology of allergic diseases

Role of IL-9 in the pathophysiology of allergic diseases
IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,
Antigen-specific effector CD8 T cells regulate allergic responses via IFN-γ and dendritic cell function  Yafang Tang, BSc, Shou Ping Guan, BSc, Benson.

Antigen-specific effector CD8 T cells regulate allergic responses via IFN-γ and dendritic cell function  Yafang Tang, BSc, Shou Ping Guan, BSc, Benson.
CD1d restricted natural killer T cells are not required for allergic skin inflammation  Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan.

CD1d restricted natural killer T cells are not required for allergic skin inflammation  Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan.

Similar presentations

Ads by Google