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CD1d restricted natural killer T cells are not required for allergic skin inflammation  Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan.

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Presentation on theme: "CD1d restricted natural killer T cells are not required for allergic skin inflammation  Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan."— Presentation transcript:

1 CD1d restricted natural killer T cells are not required for allergic skin inflammation 
Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan Scott, MD, Everett Meyer, PhD, Sho Goya, MD, PhD, Raif S. Geha, MD, Dale T. Umetsu, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 118, Issue 6, Pages (December 2006) DOI: /j.jaci Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 OVA-specific antibody levels are similar in CD1d–/– mice and WT controls after epicutaneous sensitization (EC sens.) with OVA. OVA-specific IgG1 (A), IgG2a (B), and IgE (C) antigen-specific levels were determined by ELISA. Results are expressed as means ± SDs (n = 6-8 mice per group). KO, Knockout. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Number of eosinophils (A) and CD4+ cells (B) infiltrating sensitized skin sites of CD1d–/– mice and WT controls. Results are expressed as means ± SDs; ∗∗P < .01 (n = 4-5 mice per group). EC sens., Epicutaneous sensitization; HPF, high-power fields; KO, knockout; EOS, eosinophils. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 A, Cytokine mRNA expression in sensitized skin of CD1d–/– and WT mice. Results are expressed as means ± SDs fold induction (n = 6-8). B, Representative FACS analysis of TCRβ+/α-GalCer–CD1d dimer+ cells (iNKT cells) in explanted skin cells gating on lymphocytes. C, Mean percentages ± SEMs of iNKT cells in skin explants of 4 mice per group; ∗P < .05. SAL, Saline; KO, knockout; aGC, alpha-galactosylceramide; EC sens., epicutaneous sensitization. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 Cell counts in BAL fluid from epicutaneously sensitized CD1d–/– mice and WT controls after airway challenge with OVA. Bars represent the means ± SEMs (n = 6-8 mice per group). ∗∗P < .01. SAL, Saline; KO, knockout; Macro, macrophages; Lympho, lymphocytes; Neutro, neutrophils; Eosino, eosinophils. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6 Fig 5 A, Representative FACS analysis of TCRβ+/α-GalCer–CD1d+ cells (iNKT cells) in BAL fluid gating on lymphocytes. B, Mean percentages ± SEMs of iNKT cells in the BAL fluid (n = 4). C-F, Cytokine mRNA expression in the BAL fluid of epicutaneously sensitized CD1d–/– and WT mice. Results are expressed as means ± SDs fold induction (n = 6-8); ∗P < .05. sens, Sensitized; KO, knockout. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

7 Fig 6 CD1d–/– mice epicutaneously sensitized with OVA do not develop AHR to methacholine after intranasal challenge with allergen. Data represent the means ± SEMs of enhanced pause (Penh) values (n = 6-8 mice per group). ∗P < .05. Sal, Saline; KO, knockout. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

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