1. Independent research task

2. Using the video, produce a timeline of the discovery of penicillin. Within the timeline, use the video and your knowledge to explain why some bacteria were found to be resistant to penicillin in its original form.

3. Penicillium notatum fungus 30 hours before penicillin production begins After 6 days, penicillin is extracted and purified This is batch culture fermentation

4. Penicillin is a secondary metabolite It is only produced when the glucose supply runs low Think about competition… Why would a microorganism produce microorganisms killing substances when food is low? How can we change the fermenter conditions to cause the production of penicillin?

5. Produce an A3 sheet that outlines the conditions necessary for the production of penicillin GradeWhat to do D State the most important events/milestones in the discovery of penicillin. C Describe the conditions required in the fermenter for optimal growth of a desired culture. Describe the features of a fermenter and how they maintain the conditions for fermentation. B Describe the conditions necessary for production of penicillin and how conditions in a fermenter are optimised for penicillin production. Desribe the process of making penicillin. A Deduces a biological explanation for why penicillin is produced by bacteria under low glucose conditions