1. 1st Planning Lab- The effect of testing your variable on bean plant germination & growth

2. I. Introduction p1 A.Purpose - to study the effect of a variable on seed germination (sprouting) B.Background-seeds are dormant until placed in favorable conditions including water, temperature, light, freezing, fire, oxygen, pH, soil type (sand, clay, compost)

3. C. Hypothesize or predict the result for your variable state why (state reference)

4. YOUR Mission: With your lab partners decide what environmental condition you will be testing next Wed. Write up your prelab (Part I & II of formal lab report)

5. II. A. Materials p 2 of lab notebook Clear plastic cups (100 ml) Potting soil (Miracle Grow) Water- need at least 3 ml/ 5 g of soil ~ 18 ml/cup modify for if experimental variable needs to be dissolved in water Experimental Variable- soil, temp etc Plant grow lights Plastic wrap to cover cups so they don’t dehydrate or dry out Rubber bands to secure plastic wrap Sharpies to label control vs. exp cups + period + group names

6. II. B) Method (p 2 of notebook) List the steps to do the lab as described. 1.Label cups- Table # Period; experiment or control 2. Add 70 g of potting soil /cup need at least 3 ml/ 5 g of soil ~ 70 ml/cup modify for if experimental variable needs to be dissolved in water. Too much water they drown; too little too dry to sprout. No swimming pools 3.Mix water with soil using knife- must be completely wet 4. Add 8 seeds/cup & put in 2 mm into the soil 5.Secure saran wrap with rubber band 6.Place control & exp cups under the same light source in same position under grow lights 7.Measure growth daily & water!

7. II. C Table of variable (2nd page) Independent Variable- you decide how to treat experimental group of bean seeds. –Example The experimental group will be exposed pH 3 acidic water to determine the effect of acid on seed germination

8. Table of variable hints Dependent Variable- What are you measuring?

9. Table of variable Controlled Variable- How are you making sure the control cup is the same except for the independent variable. Use full sentences to explain how & why kept the same Example- Because plant growth depends on temperature it is kept constant between control and experimental cups by placing them next to each other in the same region of classroom controlexperiment

10. III. Results put on p 3 A) Qualitative- note observations –Day 1 We accidentally spilled water and there was less in the control. –Day 3 Exp cup had no sprouting seeds. Leaves were green and healthy in the sprouting controls

11. III. Results put on p 3 of notebook B) Quantitative in table with complete title: The effect of __________ on bean plant seed germination Note units are sprouts +/- 1 sprout Germination Day 1 (sprouts) Germination Day 2 Germination Day 3 Control455 Exp.- treated to: 123

12. What is wrong with this picture? Please measure precisely w/ metrics!

13. III. Results put on p 3 of notebook B) Quantitative in table with complete title: The effect of __________ on bean plant seed growth Note units are cm +/- 1 mm Final length of sprout 1 Sprout 2Sprout 3 Add more columns if necessary… Control 4 cm +/- 1 mm 5 cm +/- 1 mm 6 cm +/- 1 mm Exp.- treated to: 1 cm +/- 1 mm2 cm +/- 1 mm3 cm +/- 1 mm

14. III. B. Data Analysis (new page) Transformed Data- use some kind of calculation- put into tables & graphs Table II- give this a complete title! % beans that germinated Average Length of bean sprouts Control80% +/- 10%10 cm +/- 1 mm Exp. (treated to:____) 10% +/- 15%6 cm +/- 1 mm

15. Write out a “ Sample Calculation ” Show how you calculated your results % germination = (# sprouts/ 5 sprouts that were planted) x100 % Uncertainty = 1/8 seeds x 100% = 12% Average length= (sum of growth)/ # of sprouts Ex ; (5 + 6 + 7)/3 = 6 cm Uncertainty + 1 mm

16. % of brine shrimp hatched Control80% +/- 10% Exp.10% +/- 15%

17. Is there significant difference here between control and exp?

18. Conclusions (new page in notebook!) Paragraph 1- A. What did you do in the experiment? How did you perform the experiment in general? What happened in the experiment? P2- B. Did the experimental condition differ frin the control? Refer to your Graph! By what %? Is this a significant difference (- look at your error bars)?

19. Conclusions (new page in notebook!) P3 C. What did you predict- restate your hypothesis. Did the results match what you predicted? Does data support or falsify your conclusion- or is your data have so much uncertainty that you can not make a conclusion. Compare to research and justify your results (look up data on web & cite it here!) This also should have been mentioned in your background.

20. Conclusions (new page in notebook!) P4 D. Explain the errors in this lab (discuss at least 3!)? What were the limitations of this lab? Were you able to obtain data accurately- if not Why? Could there be a better way? Were there outliers P5 E- for every error you wrote above- suggest a realistic way to fix the problem. For every limitation stated in the above paragraph, suggest a better way to perform this experiment.

21. Bibliography See the Biology Lab Report Guidelines I have extra copies if you lost it. Please staple to lab notebook. Follow the citation examples.

22. Formal Lab due- Friday 8.19.2016 Good Luck!