1. Lab Work 3 SDV1- Physiology II Bleeding time, clotting time and BP
Dr Than Kyaw
26 March 2012

2. Bleeding time and clotting time
Lab 3. (a and b)
Bleeding time and clotting time
Bleeding time
Aim: To determine the bleeding time“
Principle
Injury to blood vessels – bleeding
Normally bleed is stopped by endogenous homeostasis mechanism
Main involvements – vasoconstriction, temporary platelet plug formation.
followed by permanent (true) plug formation. It is a complex process
involving various clotting factors.
Clot formation
The test – mainly used before surgery or before biopsy procedure

3. Requirements: lenser, spirit swab, filter paper, stop watch/or wrist watch
Procedure
sterilize the ear lobe at with spirit.
punch using a needle or a lancer (sterile needle)
let blood flow without squeezing
Start noting the time
Mob the oozing blood at different points on the edge of a filter paper at 30 second intervals until the blood stops oozing.
Note the time at which the bleeding stop ( can also calculate by multiplying the number of blood spots with 30 seconds and converting them into minutes)
Caution: Do not press the filter paper on the bleeding spot. The skin should be sterile and dry before pricking.

5. (b) Clotting time Principle The principle is the same as
Aim: To determine the clotting time
Principle
The principle is the same as
in bleeding time.
The difference is that bleeding time assesses the primary hemostasis (platelet plug formation) and the clotting time assesses the secondary clot formation (true clot formation)

6. Requirements: Glass capillar tubes, lenser, spirit swab, filter paper, stop watch/or wrist watch
Procedure
Sterilize the finger tip with spirit and allow it to dry.
Punch the finger tip with a lancer (or a sterile needle)
Let blood flow without squeezing
Start noting the time
Introduce 2 capillaries together into the blood drop collected on the finger tip.
Blood enters the capillary tubes by capillary suction.
Make sure the capillary tubes are well filed up.
Break the tube at 30 second intervals until fibrin thread is formed
The time from pricking to fibrin thread formation is clotting time.
Precautions
similar to bleeding time.

7. Prolonged bleeding time in :
Normal values
Bleeding time to 6 minutes
Clotting time to 8 minutes
Prolonged bleeding time in :
Thrombocytopenia
Bone marrow aplisia
defective platelet aggregation
Prolonged clotting time in :
Liver diseases (production of clotting factors)
Anticoagulant therapy
Vit K deficienc

8. Format of the report DVT 1073 Fisiologi Haiwan II Lab work no. ____
Name: ____________ No. Matrik: ______________ Date: ________
Title of the laboratory work.
Aim
Requirements
Procedures
Observation and findings
Discussion/Comments/conclusion

9. The End