1. Mekanisme Kerja Hormon dr. Susila Sastri M.Biomed Biokimia FK-Unand

2. Hormone

3. Struktur Kimia Hormon 1.Polipeptida Insulin Glucagon 2.Derivat asam amino (amin) Epineprin Serotonin Tyroxin 3. Steroid (derivat kolesterol) Aldosteron Cortisol Retinoic acid 4. Eicosanoid (polyunsaturated acid, (20atom C) Prostadglandin E Thromboxane A2

4. Simple Intracellular & Extracelluler Signaling

5. Hormon Action

6. Hypophise Anterior

7. Neurohormone

8. GAS MOLECULE Cell Communication

9. Second messenger: Small molecules synthesized in cells in response to an external signal are the second messengers, which are responsible for intracellular signal transduction: Ca 2+, DG, Cer, IP 3, cAMP, cGMP

10. Receptor Receptors: specific membrane proteins, which are able to recognize and bind to corresponding ligand molecules, become activated, and transduce signal to next signaling molecules: A. Membran B. Intra Sel Glycoprotein or Lipoprotein

11. Hypothalamus Anterior pituitary Posterior pituitary Thyrotropin ACTH Somatotropin LH FSH Prolactin Vasopressin Oxytocin Thyroid Adrenal Cortex Adrenal Medulla PancreasOvary Testis Muscles liver Tissues Liver, muscles Estradiol Testosterone Insulin, glucagon, somatostatin T3 Cortisol aldosterone Mammary glands Reproductive organs Epinephrine Releasing hormones Nervous Kelenjer-Hormon-Target Sel

12. 1. Membrane receptors

13. Signal transduction mediated by membrane receptor cAMP dependent-protein kinase A pathway cGMP dependent PKG pathway Ca 2+ dependent PK pathway Tyrosine protein Kinase pathway NF-κB pathway TGF- βpathway

14. Membrane Receptor

15. Cyclic AMP

16. Mekanisme Signal Tranduksi

17. Glucagon Cyclic AMP Cyclic GMP Ca 2+ Diacylgycerol Protein substrates PK-APK-G CalmodulinPK-C Protein Ser/Thr kinases Protein substrates Multifunctional kinases Other phospholipases 1 2 3451 234 5 Tyrosine kinase IP 3 GGGG Insulin T-cell Activation Nitric oxide G protein End result is phosphorylation of one or more proteins Hormon-2 nd Massengger- Enzim

18. Inositol triphosphate System PLC; phospholipase C PIP2: phosphoinositol DAG:diacylgliserol IP3:inositol triphosphat PKC: phosphokinase C

19. Intracellular receptor (DNA transcription regulated receptor) Steroid Hormone, VD 3, Thyroxine Cytosolic R: glycocorticosteroid H Nuclear R: thyroxine, estrogen, androgen, progesterone

20. Hormon - Receptor highly specificity highly affinity saturation reversible binding special function model

21. Control of receptor activity Phosphorylation or dephosphorylation of R Phospholipid of membrane Enzyme catalyzed hydrolysis G protein regulation

23. Thyroid Mechanism of Action

24. Adrenaline Mechanism of action

25. ACTH Mechanism of Action

26. Aldosteron Mechanism of Action

27. Estrogen Mechanism of Action

28. Androgen Mechanism of Action

29. Insulin Receptor

30. Insulin Action

31. Glucagon

32. GH/ STH