1. Director, Product and Strategic Development VetEquip, Inc.

2. Why Inhalant Anesthesia?

3. 95% of US Facilities Use Inhalant Anesthesia Because… Fast Safe Cost Effective

4. Why Inhalant Anesthesia? Fast Induction < 2 minutes Recovery < 2 minutes

5. Why Inhalant Anesthesia? Safe Quickly Metabolized via Respiration Anesthetic Depth Easily Adjusted Unlikely to Overdose Subject Shorter Recovery Times

6. Why Inhalant Anesthesia? Cost Effective High Productivity Reuse of Animals for Future Study Lower Mortality Rate Anesthetic Agent Relatively Low Cost No Drug Logs/Special Licenses

7. Anesthesia Systems One or Two Animals

8. Anesthesia Systems Multiple Animals/High Volume

9. Special Applications Molecular Imaging

10. Molecular Imaging Considerations Compatible With All Modalities Animal Handling Imaging Facility Layout Single vs. Multi Modality Imaging Biosafety and Radiation

11. Molecular Imaging Solutions Core Anesthesia System Operate Multiple Devices in Separate Areas Local Control of Devices Simple Operation

12. Molecular Imaging Solutions Animal Handling Heated Induction Chambers Multi Modality Bed Optical Manifold Custom Stacked Bed

13. Anesthesia System Maintenance Definitions Service Preventative Maintenance Efficacy Test Calibration Certification

14. Anesthesia System Maintenance Definitions Service Performed “As Needed” Unscheduled Maintenance

15. Anesthesia System Maintenance Definitions Preventative Maintenance Annual Inspection of System and Components Efficacy Testing of Anesthetic Vaporizer

16. Anesthesia System Maintenance Definitions Efficacy Test Vaporizer Output Verification Done at High and Low Flow Rates and Anesthetic Concentrations Part of Annual P/M

17. Anesthesia System Maintenance Definitions Calibration Full Vaporizer Service Performed Every 3 Years or Failure of Efficacy Testing or Manufacturer Recommendations

18. Anesthesia System Maintenance Definitions Certification P/M Documentation Guarantee of Proper Performance for Certification Period (1 Year)

19. Anesthesia System Maintenance

21. GLP Considerations USA Environmental Protection Agency Food & Drug Administration International Organization for Economic Cooperation & Development (OECD)

22. GLP Considerations US FDA and EPA Tend to have more requirements than OECD Require SOPs for Equipment Maintenance and Calibration

24. GLP Considerations FDA/EPA/OECD Overriding Principles State What You’re going to Do Do What You Stated You’d Do Document That You Did It

25. Thank You 감사합니다