1. Bioanalytik detect and identify Over 25 years experience in Imaging Benefits of NightOWL

2. Bioanalytik detect and identify In 1989 BERTHOLD TECHNOLOGIES developed the first low level light imager In 1993 BERTHOLD stated in its brochure to do non-invasive bioluminescent in-vivo-imaging In 1996 NightOWL became introduced

3. Bioanalytik detect and identify NC 100>-80°C cooled backlit CCD camera 1 M pixel; 1024 x 1024 pixel; 1:1 13 x 13 µm pixel size dr 1 : 50.000 (best for in-vivo imaging) NC 320>-60°C cooled frontlit CCD camera 3.2 M pixel; 2154 x 1640 pixel; 1,3:1 6,8 x 6,8 µm pixel size dr 1 : 8.000 (best for fluorescence) No PC-board anymore. Connecting via Ethernet! NightOWL benefits (1) Interchangeable Cameras

4. Bioanalytik detect and identify 10 luminescent cells in total are detectable in a microplate well. NightOWL benefits (1) Sensitive Cameras

5. Bioanalytik detect and identify 1000 luminescent cells in total or 20 cells per mm² subcutaneously in a mouse are easy detectable. Detection limit is 150 – 250 cells in a cluster. NightOWL benefits (1) Sensitive Cameras

6. Bioanalytik detect and identify Easy setting of sample size and height Easy setting of acquisition time from 30 ms up to hours. Analysis is possible via Line plot Threshold 3D-plot ROI manually Edit functions Color overlay Pseudo colors Multicolor overlay NightOWL benefits (2) WinLight – Powerful scientific software for NightOWL (1)

7. Bioanalytik detect and identify Everything is defined by the user Flatfield correction Height correction in each camera position Cosmic suppression Background substraction Arithmetric functions Geometrical Analysis (area, length etc.) Quantitative Analysis Same camera position Same exposure time Same threshold Sequence measurements and Analysis Macro function for full automated image analysis NightOWL benefits (2) WinLight – Powerful scientific software for NightOWL (2)

8. Bioanalytik detect and identify Pixel are combined (“binned”) to superpixel indepentently. Per readout process one readout noise is generated, independent whether a single pixel or a superpixel is read out. Pixel binning increases the s/n ratio: one readout noise and a big signal Loss of spatial resolution. NightOWL features (3) User defined pixel binning

9. Bioanalytik detect and identify NightOWL benefits (4) Moving camera and Working distance 3,5 cm 75 cm Focus (cm)Resolution (µm) 3.5 50 75320

10. Bioanalytik detect and identify NightOWL benefits (5) Power sockets and connectors inside for individual set-ups like Extra lamps Cooler or heater ?

11. Bioanalytik detect and identify NightOWL benefits (6) Flange option with the flange option additional in- and out-lets are possible For gas For hot water For fibre optics For electric cables

12. Bioanalytik detect and identify NightOWL benefits (7) Macro table Distance is important

13. Bioanalytik detect and identify NightOWL benefits (7) Macro table Gooseneck dsRed fluorescence

14. Bioanalytik detect and identify C-mount adapter Via C-mount adapter the NightOWL cameras can be easily attached to each microscope with C-mount port. BERTHOLD offers a specific microscope option, so that only 2 thumb screws has to be unscrewed. NightOWL benefits (8)

15. Bioanalytik detect and identify NightOWL benefits (9) Fluorescence Sophisticated lamp control Constant lamp beam via Photodiode control Lamp factor can be set to calibrate the system.

16. Bioanalytik detect and identify 3 modells of illumination are available Ringlight epi illumination Dual-line epi illumination Gooseneck spot illumination Filter slide for emission 90 W tungsten light source Filters available from 200 - 1100 nm NightOWL benefits (9) Flexible fluorescence epi-illumination

17. Bioanalytik detect and identify NightOWL benefits (9) GFP fluorescence and NIR-fluorescence Ex.: 680 nm Em.: 720 nm

18. Bioanalytik detect and identify Transilluminators with 20 x 20 cm illuminated area 8 lamps with wavelenghts of 256 nm 312 nm 365 nm Blue White + converters NightOWL benefits (9) Full range of fluorescence trans-illumination

19. Bioanalytik detect and identify Via the flange option the anesthesia gas flow is directed in and out. Inside up to 5 rodents can be anesthesized. NightOWL benefits (10) Gas anaesthesia system (1)

20. Bioanalytik detect and identify Gas flow meters Vaporizer Knock-out box Flange option with gas in- and outlet NightOWL benefits (10) Gas anaesthesia system (2)

21. Bioanalytik detect and identify NightOWL benefits (11) Orthogonal 3D-option With such a mirror system images from top, left and right can be obtained. Measuring the lenght, width and depth of the light source, volume calculation can be done with r 2 = 0,89.

22. Bioanalytik detect and identify NightOWL benefits (11) Orthogonal 3D-option In the overlay images you can use the line-plot function to measure length, width (from top-image) and height (from the right image) to calculate the volume according to the simple formula L x W x H / 2.

23. Bioanalytik detect and identify NightOWL benefits (12) To perform experiments with different imaging technologies, a carrier for MRI and PET was developed by medres. MACU = Multimodal Animal Carrier Unit

24. Bioanalytik detect and identify Over 25 years experience in Imaging Thanks for Your attention!