1. M O S T P R O B A B L E N U M B E R

2. ( Sutton, 2010 ) ( USP, 2007 )

3. Mc Crady Cochran Woodward De Mann

4. MO distributed through the sample 1 MO separate individually not in chain 2

5. Media is suitable to growing MO 3 Just show amount of viable MO 4 SIMULATIONS

6. SAMPLE 1 ml Durham’s Tube Prepare the sample and dilution series 1 10 -1 10 -2 10 -3

7. Incubated 24 Hours 2

8. Gas production Indicate positive result Identify Positive Tube 3

9. 321 Count Positive Tube 4

10. Convert to MPN Tabel 5

11. Cochran, W. 1950. Estimation of Bacterial Densities by Means of the Most Probable Number. Biometrics 6:105-116. Sutton, S. 2010. The Most Probable Number Method and Its Uses in Enumeration, Qualification, and Validation Journal of Validation Technology Vol. 16(3): 35-38. USP, Chapter. 2007. Microbiological Control and Monitoring Environments Used for the Manufacture of Healthcare Products. Pharm Forum 33(3).