1. Date of download: 6/2/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Biological and Ultrastructural Properties of Acelagraft, a Freeze-Dried γ-Irradiated Human Amniotic Membrane Arch Ophthalmol. 2010;128(10):1303-1310. doi:10.1001/archophthalmol.2010.222 Smoothness of decellularized and dehydrated human amniotic membrane (DDHAM) evaluated by scanning electron microscopy (A and B) and by atomic force microscopy (C-F). A, Low magnification (original magnification ×18; bar = 1 mm) of Acelagraft (Acelagraft Cellular Therapeutics, Cedar Knolls, New Jersey). B, High magnification (original magnification ×500; bar = 50 μm) showing smoothness of DDHAM surface. C, Height data of DDHAM where dark areas represent depressions and brighter areas represent protrusions on the surface. D, Amplitude data. E, Phase data depicting hardness variations. Bright areas represent hard regions whereas dark areas represent soft regions on the surface. F, 3-Dimensional presentation of topographical map. Image scale: 50 × 50 μm. Figure Legend:

2. Date of download: 6/2/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Biological and Ultrastructural Properties of Acelagraft, a Freeze-Dried γ-Irradiated Human Amniotic Membrane Arch Ophthalmol. 2010;128(10):1303-1310. doi:10.1001/archophthalmol.2010.222 Slitlamp photography and fluorescein staining patterns of the clinical subject taken preoperatively (A), 1 day after surgery (B), and 17 days after surgery (C). Figure Legend:

3. Date of download: 6/2/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Biological and Ultrastructural Properties of Acelagraft, a Freeze-Dried γ-Irradiated Human Amniotic Membrane Arch Ophthalmol. 2010;128(10):1303-1310. doi:10.1001/archophthalmol.2010.222 Cross-section ultrastructural comparison between the basement membrane of cryopreserved human amniotic membrane (CHAM) (A) and decellularized and dehydrated human amniotic membrane (DDHAM) (B) tissue. A, Transmission electron micrograph of CHAM tissue. E indicates epithelium; LD, lamina densa; S, stroma (original magnification ×30 000). B, High magnification of transmission electron micrograph of DDHAM tissue (original magnification ×30 000). Inset: Low magnification showing DDHAM tissue consists of thick basement membrane (arrow) and an avascular stroma (S) (original magnification ×12 000; bar = 1 μm). Figure Legend:

4. Date of download: 6/2/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Biological and Ultrastructural Properties of Acelagraft, a Freeze-Dried γ-Irradiated Human Amniotic Membrane Arch Ophthalmol. 2010;128(10):1303-1310. doi:10.1001/archophthalmol.2010.222 Representative images of Western blot analysis of fibronectin; collagen types IV and VI; laminin 5; and elastin of cryopreserved human amniotic membrane (CHAM) and decellularized and dehydrated human amniotic membrane (DDHAM) (Acelagraft; Acelagraft Cellular Therapeutics, Cedar Knolls, New Jersey). Figure Legend:

5. Date of download: 6/2/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Biological and Ultrastructural Properties of Acelagraft, a Freeze-Dried γ-Irradiated Human Amniotic Membrane Arch Ophthalmol. 2010;128(10):1303-1310. doi:10.1001/archophthalmol.2010.222 Immunohistochemical staining of various extracellular matrix and growth factor proteins in cryopreserved human amniotic membrane (CHAM) and decellularized and dehydrated human amniotic membrane (DDHAM) (original magnification ×200). EGFR indicates epidermal growth factor receptor; FGF-2, fibroblast growth factor 2; IGF-I, insulinlike growth factor I; PDGF, platelet- derived growth factor; TGF, transforming growth factor; VEGF, vascular endothelial growth factor; +++, strong staining; ++, moderate staining; +, weak staining; −, negative staining. Figure Legend:

6. Date of download: 6/2/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Biological and Ultrastructural Properties of Acelagraft, a Freeze-Dried γ-Irradiated Human Amniotic Membrane Arch Ophthalmol. 2010;128(10):1303-1310. doi:10.1001/archophthalmol.2010.222 Hematoxylin-eosin staining of cryopreserved human amniotic membrane (A) and decellularized and dehydrated human amniotic membrane (Acelagraft; Acelagraft Cellular Therapeutics, Cedar Knolls, New Jersey) (B) (original magnification ×400). Figure Legend: