1. Gene Expression Ilana Granovsky Jonathan Laserson

2. Yeast Cluster http://rana.stanford.edu/

3. Our Cluster

4. Finding the Common Ground Resemblance in DNA sequence Resemblance in protein sequence Regulatory motifs Conserved regions in proteins Protein structure

5. Tools Used BLAST CLUSTALW PSI-BLAST MEME MOTIF PSIPRED

6. Sequence Alignment No significant similarity was found

7. Protein Pairwise BLAST 7 proteins make 21 possible pairs. Only few pairs showed resemblance, with very high expectancy.

8. Regulatory Motifs We used MEME to find motifs in the DNA sequence prior to the beginning of each gene. Result: no motifs were found.

9. Regulatory Motifs We used MEME to find motifs inside each gene.

10. Motif Description 1.Cap signal for transcription initiation 2.Heat shock factor http://www.motif.genome.ad.jp/

11. Conserved Regions in Proteins No motifs were found which shared more than 2 sequences Attempts to divide the proteins to distinct sub-groups also brought no improvement

12. Secondary Structure No apparent similarity was found

13. Conclusions Similarity was found in DNA sequence motifs These motifs allow rapid transcription of the genes in specific situations No other similarity was found

14. Future Analysis There is no alternative to lab experiments!

15. thanks To Varda