1. CYSTIC FIBROSIS ALBINISM HEMOPHILIA DWARFISM WHAT DO THEY HAVE IN COMMON? THEY ARE GENETIC DISEASES (DISORDERS) http://radiopaedia.org/ https://commons.wikimedia.org http://discovermagazine.com/

2. GENES, MUTATIONS & DISEASES: UNDERSTANDING THE ORIGINS OF GENETIC DISORDERS http://www.raogk.org/dna/

3. SICKLE-CELL ANEMIA IS A GENETIC DISEASE http://geneed.nlm.nih.gov/

4. LESSON #1

5. genetic gene - fundamental unit of heredity - set of instructions, typically to make a protein - composed of deoxyribonucleic acid (DNA) DNA chromosome nucleus

6. gene DNA - nucleic acids are composed of nucleotides - DNA is composed of two strands of nucleic acids nucleotide http://igbiologyy.blogspot.com/ http://www.livescience.com/

7. sugar phosphate base Four different bases give rise to four different nucleotides in DNA. -bases: adenine (A), guanine (G), cytosine (C), and thymine (T) -pairing of bases: A with T G with C A T AT G C DNA http://www.livescience.com/

8. Phosphate groups (therefore, DNA molecules too) carry negative charges. Base-pairing occurs through hydrogen bonds. http://dnarnanews.blogspot.com/ http://knowgenetics.org/

9. Break cells open and precipitate DNA using extraction buffer. When DNA comes out of solution it tends to clump together, which makes it visible. You will take the clump, re-suspend it in water and run a gel to see if it actually is DNA. As comparison, you will also run samples of water and protein. ACTIVITY #1 EXTRACTING AND VISUALIZING DNA https://commons.wikimedia.org

10. Power Source OFFON Ladder #1 #2 #3 #4 1200 bps 500 bps 250 bps 125 bps 100 bps ( - ) ( + ) Agarose Gel Electrophoresis A DNA ladder is added so you can determine the size of your DNA fragments Electrophoresis ●Uses an electrical current to separate molecules with different charges Agarose ●Used separate molecules by shape and size Agarose Gel Electrophoresis ●Pulls molecules through an agarose gel using an electrical current

11. ACTIVITY #2 MAKING A DNA MODEL Models are used by scientists to describe ideas, understand biological processes and make predictions. Each bench has one strand of DNA. Build the complementary strand using the free nucleotides at your bench. The magnetic bars represent the hydrogen bonds. After building your model, twist the strands.