Gene Expression Profile in Proliferation and Apoptosis of Human Hepatic Stellate Cell Using Microarray 신혜원 병리학교실

Introduction Hepatic fibrosis is one of the common disease in Koreans. Preventing initial liver fibrogenesis may contribute to hepatic fibrosis therapies. It is important to discover the mechanisms related to the proliferation and apoptosis of hepatic stellate cell line which has the critical role in liver fibrogenesis.

AIM To compare gene expression patterns time dependently between normal, activated and apoptotic human hepatic stellate cell line To identify critical genes involved in hepatic fibrosis

Materials & Method hTERT cell line : immortalized activated human hepatic stellate cell line PDGF-BB : Platelet Derived Growth Factor-BB ; a potent growth factor Curcumin : a phytochemical compound known to induce apoptosis

PDGF-BB and curcumin treatment on hTERT cell line mRNA extraction of proliferation and apoptosis induced hTERT cell line Microarray hybridization

Data acquisition Spot preprocessing, quality inspection, normalization, clustering & classification, differentially expressed gene finding & biological validation Gene expression confirmation by RT-PCR