What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. ≈160,000.

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Presentation transcript:

What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. ≈160,000 bp in bacteria ≈100,000,000 bp in eukaryotes ≈3,000 bp

Plasmid Structure

Reporter genes

GFP Tagging

Recombinant Gene Expression Media includes dissolved iron and Sulfuric acid

Acid Mine Drainage

Protein Product Purification

Antibiotic Selection

Recombinant DNA Responsibility Sex in the Sink: Gene-Swapping Bacteria Are Making New Superbugs -NBC News