© 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.

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Presentation transcript:

© 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence

© 2015 BioLife Solutions, Inc. 2 5-Day Hypothermic Storage of Human Dermal Fibroblasts Enabled by HypoThermosol ® alamarBlue ® stain for metabolic activity * Contains serum % Recovery of Viable Cells Assayed 24 Hours Post Storage Note: The following slide is a better visual display of this information, but it is fixed art, and cannot be edited. So this slide, which is editable is being kept in this deck as a hidden slide, in case future changes are needed.

© 2015 BioLife Solutions, Inc. 5-Day Hypothermic Storage of Human Dermal Fibroblasts Enabled by HypoThermosol ® alamarBlue ® stain for metabolic activity * Contains serum Assayed 24 Hours Post Storage - % Recovery of Viable Cells DAY STORAGE 1 3 5

© 2015 BioLife Solutions, Inc. 4 Representative Fluorescent Micrographs Illustrating Morphology of Normal Human Dermal Fibroblasts Stored in HypoThermosol ® Green Actin Cytoskeleton Phalloidin (FITC) Red Mitochondria Activation MitoTracker® Red Blue Nuclear Stain Hoechst 40X magnification Media with Serum 3 Day Storage 5 Day Storage Celsior ® 3 Day Storage 5 Day Storage Viaspan ® 3 Day Storage 5 Day Storage HypoThermosol ® 3 Day Storage 5 Day Storage

© 2015 BioLife Solutions, Inc. 5 alamarBlue ® stain for metabolic activity * Contains serum Extended Hypothermic Storage of Human Mesenchymal Stem Cells Enabled by HypoThermosol ® Assayed 24 Hours Post Storage Note: The following slide is a better visual display of this information, but it is fixed art, and cannot be edited. So this slide, which is editable is being kept in this deck as a hidden slide, in case future changes are needed. % Recovery of Viable Cells

© 2015 BioLife Solutions, Inc. 6 alamarBlue ® stain for metabolic activity * Contains serum Extended Hypothermic Storage of Human Mesenchymal Stem Cells Enabled by HypoThermosol ® Assayed 24 Hours Post Storage - % Recovery of Viable Cells DAY STORAGE

© 2015 BioLife Solutions, Inc. 7 Representative Fluorescent Micrographs Illustrating Morphology of Human Mesenchymal Stem Cells Stored in HypoThermosol ® Green Actin Cytoskeleton Phalloidin (FITC) Red Mitochondria Activation MitoTracker® Red Blue Nuclear Stain Hoechst 20X magnification Normosol ® -R 3 Day Storage 5 Day Storage Plasma ® -Lyte 3 Day Storage 5 Day Storage Viaspan ® 3 Day Storage 5 Day Storage HypoThermosol ® 3 Day Storage 5 Day Storage

© 2015 BioLife Solutions, Inc. 8 alamarBlue ® stain for metabolic activity * Contains serum Recovery of Human Osteoblast Cells Following Hypothermic Storage Assayed 24 Hours Post Storage Differentiated Following 5-Day Hypothermic Storage of Undifferentiated hMSC in HypoThermosol ® % Recovery of Viable Cells Note: The following slide is a better visual display of this information, but it is fixed art, and cannot be edited. So this slide, which is editable is being kept in this deck as a hidden slide, in case future changes are needed.

© 2015 BioLife Solutions, Inc. 9 Recovery of Human Osteoblast Cells Following Hypothermic Storage Assayed 24 Hours Post Storage Differentiated Following 5-Day Hypothermic Storage of Undifferentiated hMSC in HypoThermosol ® Assayed 24 Hours Post Storage - % Recovery of Viable Cells DAY STORAGE alamarBlue ® stain for metabolic activity * Contains serum

© 2015 BioLife Solutions, Inc. 10 Representative Fluorescent Micrographs Illustrating Morphology of Human Osteoblast Cells Following Hypothermic Storage 20X magnification Differentiated Following 5 Day Hypothermic Storage of Undifferentiated hMSC in HypoThermosol Green Actin Cytoskeleton Phalloidin (FITC) Red Mitochondria Activation MitoTracker® Red Blue Nuclear Stain Hoechst Normosol ® -R 3 Day Storage 6 Day Storage Plasma ® -Lyte 3 Day Storage 6 Day Storage Viaspan ® 3 Day Storage 6 Day Storage HypoThermosol ® 3 Day Storage 6 Day Storage

© 2015 BioLife Solutions, Inc. 11 alamarBlue ® stain for metabolic activity * Contains serum 24 Hour Post Storage Recovery of Human Dental Pulp Stem Cells Following Hypothermic Storage Commercial Solution Comparison: Serum and Serum-Free Media % Recovery of Viable Cells Note: The following slide is a better visual display of this information, but it is fixed art, and cannot be edited. So this slide, which is editable is being kept in this deck as a hidden slide, in case future changes are needed.

© 2015 BioLife Solutions, Inc Hour Post Storage Recovery of Human Dental Pulp Stem Cells Following Hypothermic Storage Commercial Solution Comparison: Serum and Serum-Free Media DAY STORAGE % Recovery of Viable Cells alamarBlue ® stain for metabolic activity * Contains serum