Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister

Slides:

Advertisements

Similar presentations

The «Lab-On-a-Chip» Concept Initiated in the 1990’s, Lab-On-a-Chip (LOC) technology represents a revolution in laboratory experimentation. The main benefits.

Advertisements

RESULTS A. ENDOTHELIAL CELL ADHESION Both attachment time and culture glucose conditions affect endothelial cell adhesion. MATERIALS & METHODS A. ENDOTHELIAL.

John D. Williams, Wanjun Wang Dept. of Mechanical Engineering Louisiana State University 2508 CEBA Baton Rouge, LA Producing Ultra High Aspect Ratio.

Microfluidic Valve Innovation Jo Falls Porter, RET Fellow 2009 West Aurora High School RET Mentor: Dr. David T. Eddington, PhD NSF- RET Program Introduction.

MONOLITHIC 3-D MICROFLUIDIC DEVICE FOR CELL ASSAY WITH AN INTEGRATED COMBINATORIAL MIXER 陳睿鈞 Mike C. Liu, Dean Ho, Yu-Chong Tai Department of Bioengineering,

Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography EECE 491C Unger et al. (Quake group, CALTECH) Science, 2000.

Final report Department ： Institute of NEMS Student ID ︰ d Report ︰ Yen - Liang Lin.

Maastricht, January 25-29, MEMS 2004 Website: Plastic Micropump using Ferrofluid and Magnetic Membrane Actuation C. Yamahata and M.

Microbubble Generation for Medical Applications Shirley Zhang Faculty Mentor: Abraham Lee Graduate Student: Kanaka Hettiarachchi.

A PDMS DIFFUSION PUMP FOR ON-CHIP FLUID HANDLING IN MICROFLUIDIC DEVICES Mark A. Eddings and Bruce K. Gale Department of Bioengineering, University of.

Gas Exchange in Lungs Prof. K. Sivapalan. Properties of Gases 20132Gas Exchange.

Basic Silicone Chemistry (II)

Microfluids ENGR Lecture. Learning Objectives of Lab  Understand capillary flow and how a capillary valve works.  Explore how the flow of fluid.

Connectors 13/03/2013Collaboration meeting CERN - CSEM1.

Development of a Modular Peristaltic Microfluidic Pump and Valve System 3/13/2007 BME 273 Group 20: Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister.

Device Design: Stage 2 (Modified Microchannel Design) Device Objective –To test the viability of a two-level passive micro-fluidic device Modifications.

Microfluidic System for Automatic Cell Culture Chun-Wei Huang, Song-Bin Huang, Gwo-Bin Lee Department of Engineering Science, National Cheng Kung University,

BGU Physics Department: Going with the (Laminar) Flow

Intro to Lab 3: Modeling a Microvascular Network on a Chip

指導老師:許藝菊學生:邱建龍介電電泳（DEP）基於微流體粒子分離器Dielectrophoresis (DEP) Based Microfluidic Particle Separator.

 Idea: To characterize a simple model of the microvascular system using a microfluidic device  Potential Uses:  Simulate clot, stroke, plaque buildup.

Flow cell design for WGM optical biosensor Yongqiang Yang BE8280 spring 2015.

Development of a Modular Microfluidic Peristaltic Pump and Valve System Jacob Hughey ¶, Matt Pfister ¶, Adam Dyess ¶, Michael Moustoukas ¶ Advisor: John.

Multicellular organisms need transport systems to deal with surface area to volume ratio issue. Animal transport and exchange systems In mammals, nutrients,

HYBRID ROCKET PROPULSION SYSTEMS BY: MCDOG SNIZZY ALLEN A.K.A SHAWN.

Instrumented NanoPhysiometer for High Throughput Drug Screening D. Michael Ackermann, Jon Payne, Hilary Samples, James Wells.

Microfluidic formation of lipid bilayer array for membrane transport analysis Sadao Ota, Wei-Heong Tan, Hiroaki Suzuki, and Shoji Takeuchi Center for International.

CELL-ENVIRONMENT INTERACTION (OUTSIDE-IN) Yuan Liu.

Device Design: Stage 2 (Modified Microchannel Design) Device Objective –To test the viability of a two-level passive micro-fluidic device Modifications.

Microfluidics Design & Chip Application Reporter: AGNES Purwidyantri Student ID no: D Biomedical Engineering Dept.

Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS) Team Members: Allyson Fry Bryan Gorman Jonathan Lin William Wong Advisor: Dr. John P.

Development of a Modular Peristaltic Microfluidic Pump and Valve System 2/13/2007 BME 273 Group 20: Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister.

BioMEMS Device Integration, Packaging and Control for BioMEMS

Stage st Trial Problems Thickness of PDMS layers Interconnects Delamination Air bubbles.

Optimization of T-Cell Trapping in a Microfluidic Device Group #19 Jeff Chamberlain Matt Houston Eric Kim.

Development of a Modular Peristaltic Microfluidic Pump and Valve System 1/30/2007 BME 273 Group 20: Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister.

Fully-integrated microfluidic chips capable of performing DNA amplification from RNA virus, sample transportation, capillary electrophoresis separation,

Introduction Abstract Results and Analysis Conclusions Materials and Methods Acknowledgements Microfluidic devices allow for high throughput experimentation.

Movement Through The Cell Membrane. How Things Move in and Out of the Cell The cell membrane is selectively permeable, allowing some substances, but not.

Electrochemical Bubble Valves Center for Bio-Mems,State University of NewYork at Buffalo.

Micro-fluidic Applications of Induced-Charge Electro-osmosis

Harmful algal bloom (HAB)-on-a-chip: Development of a microfluidic platform to study algal chemotaxis Kelly O’Quinn Dr. Adam Melvin Cain Department of.

Instrumented NanoPhysiometer for High Throughput Drug Screening D. Michael Ackermann, Jon Payne, Hilary Samples, James Wells.

Multilayer Microfluidics ENMA490 Fall 2003 Brought to you by: S. Beatty, C. Brooks, S. Dean, M. Hanna, D. Janiak, C. Kung, J. Ni, B. Sadowski, A. Samuel,

Optimization of T-Cell Trapping in a Microfluidic Device Group #19

MICROCHANNEL DESIGN ISSUES Susan Beatty Anne Samuel Kunal Thaker.

Multilayer Microfluidics ENMA490 Fall 2003 Brought to you by: S. Beatty, C. Brooks, S. Dean, M. Hanna, D. Janiak, C. Kung, J. Ni, B. Sadowski, A. Samuel,

Multilayer Microfluidics ENMA490 Fall 2003 Brought to you by: S. Beatty, C. Brooks, S. Dean, M. Hanna, D. Janiak, C. Kung, J. Ni, B. Sadowski, A. Samuel,

Lab-on-Chip Workshop March 25, 2016 Eric Johnston Soft Lithography Manager Quattrone Nanofabrication Facility.

Topic 7/8 – Hydraulics and Pneumatics Hydraulic systems use the force of a liquid in a confined space. Hydraulic systems apply two essential characteristic.

Microfluidic generator of sub-10-micron hydrosomes Zhenghao Ding, Lunjun Liu, Gabriel C. Spalding* Physics Department, Illinois Wesleyan University Abstract.

Microfluidic components 2016

Date of download: 6/27/2016 Copyright © 2016 SPIE. All rights reserved. (a)Three-dimensional (3-D) model of the air amplifier where d1 and d2 are the depth.

Multilayer Microfluidics ENMA490 Fall 2003 Brought to you by: S. Beatty, C. Brooks, S. Dean, M. Hanna, D. Janiak, C. Kung, J. Ni, B. Sadowski, A. Samuel,

Bharath S. Kattemalalawadi interfacial Science and Surface Engineering Lab (iSSELab) Department of Mechanical Engineering, University of Alberta, Edmonton,

Date of download: 9/18/2016 Copyright © ASME. All rights reserved. From: Droplet Detachment Mechanism in a High-Speed Gaseous Microflow J. Fluids Eng.

Utah Nanofab Design Review Meeting Device Architecture (Top View Layout and Layer Cross Section) Recipes & Settings Standard Concept Equipment & Tools.

A Glance at microvalves

SELF-FOLDING TECHNIQUE

Architecture Synthesis for Cost Constrained Fault Tolerant Biochips

Science 8 - Unit A - Mix and Flow of Matter

3-Valve Pumping Sequence 4-Valve Pumping Sequence

Pressure Actuated Valve Test Design

Shawna Dean October 28, 2003 ENMA 490

Applications and Acknowledgements

Gripper that grasps autonomously.

Soft, bistable valve acting as a pneumatic switch.

Fig. 2 Fluidic and electrical characteristics of the wireless optofluidic system. Fluidic and electrical characteristics of the wireless optofluidic system.

Fig. 3 Scheme of the system connected to the microfluidic chamber.

Presentation transcript:

Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister Development of a Modular Peristaltic Microfluidic Pump and Valve System BME 272 Group 20: Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister

Microfluidics Minimal reagent consumption Increased speed of reactions Study of biological phenomena at the single cell level

Soft Lithography

Current Pumps at VIIBRE Harvard Pico Plus syringe pumps $2,000 / pump Limiting complexity of microfluidic devices

Pneumatic Valves Two-layer PDMS device Flow layer (bottom) control layer (top) Thin PDMS membrane deflects into the flow channel when the control channel is pressurized Low aspect ratios 1:20 for rectangular control channel 1:10 for round flow channel Unger et al. 2000

Pneumatic Valve Design Flow channel dimension – 100um wide, 10um tall (round, AZ p4620, mask) or 5um tall (rectangular, SU8) Control channel – valve area (300um by 300um), control line 100um wide, 25um-30um tall

Valve in Action

Current Design of Peristaltic Pump Consists of 4 valves Round flow channel Spacing between each valve 100um Control pressure: 20psi-25psi Issues Mechanical stability of membrane Reusability of pumps Surface adsorption to PDMS Flow rates and pressures unknown Gas diffusion through membrane Minimize peristalsis

Pump in Action

Nitrogen or Liquid Screw or Pneumatic Valves Christmas Tree Nanophysiometer Connector Fluid Flow Peristaltic Pump

Future Work Learn microfabrication procedures from Yuxin Get Mike and Adam trained in microfabrication (pending IRB approval) Extension and documentation of LabVIEW pump control system Build additional valve control box Characterize valves and pump