Enzymes https://www.youtube.com/watch?v=6Nw6XOqKuWg HOW ENZYMES WORK..

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Presentation transcript:

Enzymes https://www.youtube.com/watch?v=6Nw6XOqKuWg HOW ENZYMES WORK.. 1:51 MIN.

What Are Enzymes? Most enzymes are Proteins (tertiary and quaternary structures) Act as Catalyst to accelerates a reaction Not permanently changed in the process

Enzymes Are specific for what they will catalyze Are Reusable End in –ase -Sucrase -Lactase -Maltase

How do enzymes Work? Enzymes work by weakening bonds which lowers activation energy

Enzymes Without Enzyme With Enzyme Free Energy Progress of the reaction Reactants Products Free energy of activation

Enzyme-Substrate Complex The substance (reactant) an enzyme acts on is the substrate Enzyme Joins Substrate

Active Site A restricted region of an enzyme molecule which binds to the substrate. Active Site Enzyme Substrate

Induced Fit A change in the shape of an enzyme’s active site Induced by the substrate

Induced Fit A change in the configuration of an enzyme’s active site (H+ and ionic bonds are involved). Induced by the substrate. Enzyme Active Site substrate induced fit

What Affects Enzyme Activity? Three factors: 1. Environmental Conditions 2. Cofactors and Coenzymes 3. Enzyme Inhibitors

1. Environmental Conditions 1. Extreme Temperature are the most dangerous - high temps may denature (unfold) the enzyme. 2. pH (most like 6 - 8 pH near neutral) 3. Ionic concentration (salt ions)

2. Cofactors and Coenzymes Cofactors: Inorganic substances (zinc, iron) and vitamins (respectively) are sometimes need for proper enzymatic activity. Ex. Iron must be present in the quaternary structure - hemoglobin in order for it to pick up oxygen. Coenzimes: Organic substances are sometimes need for proper enzymatic activity. Ex, Vitamin B12

Two examples of Enzyme Inhibitors a. Competitive inhibitors: are chemicals that resemble an enzyme’s normal substrate and compete with it for the active site. Enzyme Substrate Competitive inhibitor

Inhibitors b. Noncompetitive inhibitors: Inhibitors that do not enter the active site, but bind to another part of the enzyme causing the enzyme to change its shape, which in turn alters the active site. Enzyme Noncompetitive Inhibitor Substrate active site altered

THE ROLE OF ENZYMES. https://www. youtube. com/watch THE ROLE OF ENZYMES https://www.youtube.com/watch?v=myORDWVzNhc 3:35 MIN. Enzymes..animated https://www.youtube.com/watch?v=tI69AVRW0DU 4:46 min. “how enzymes work..1;18..animated https://www.youtube.com/watch?v=r1ryDVgx0zw