Enrichment products for rotifers

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Presentation transcript:

Enrichment products for rotifers Live food aquaculture training course www.aquatrain.org

Enrichment of rotifers Essential fatty acids eicosapentaenoic acid (EPA 20:5n-3) docosahexaenoic acid (DHA 22:6n-3) Arachidonic acid (ARA 20:4n-6) Proteins and essential amino acids Vitamins Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Essential Fatty Acids Eicosapentaenoic acid (EPA 20:5n-3), improves survival Docosahexaenoic acid (DHA 22:6n-3) accumulating in the brain of larval fish, improves pigmentation of flat fish, improves growth and development. Arachidonic acid (ARA 20:4n-6) substrate for producing eicosanoids 16:0n-9 and 18:1n-9 promote growth Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Enrichment with HUFAs Rotifers incorporate HUFAs within a few hours Rotifers are non selective for uptake and non selective for catabolism so can be enriched with lipids on a short term protocol but there is a danger that they can have too high lipid content. Live food aquaculture training course www.aquatrain.org

Vitamin C Ascorbic acid Vitamin C can assist with stress tolerance and reduce deformity (opercular plate for bream) Rotifers fed bakers yeast attain 150 mg/g -1 DW Chlorella attain 1000 to 23000 mg/g-1 DW (depending on algal quality) Ascorbyl palmate is accumulated by the rotifer and converted to free AA. 5% AP w/w in emulsion is converted into AA concentration of 1700 mg/g-1 DW AA incorporated in the rotifer remains at high levels even after 24 hours in storage Live food aquaculture training course www.aquatrain.org

Ascorbic Acid and rotifers Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Enrichment with Vit C Enrichment using ascorbyl palmitate. AP is converted by the rotifers into active Ascorbic Acid up to 1700 mg.g-1DW after 24 h enrichment when using a 5 % AP (w/w) emulsion Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Proteins Protein content of rotifers vary between 28 and 67% of dry weight. Variability is dependant on nutritional status Protein:lipid ratio is positively correlated to SGR with fast growing rotifers (high protein to lipid ratios) giving better larval growth and survival (Turbot). Protein and has great affect on early larval production success. Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Phospolipids used for the formation of cell components particularly during rapid growth periods during rapid growth phospolipids probably not produced fast enough to meet requirements found to reduce malformation in larvae Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Algae Nannochloropsis rich in EPA and ARA Isochrysis rich in DHA Rotifers enriched with Isochrysis will attain a DHA:EPA ratio of 2 within a few hours. Live food aquaculture training course www.aquatrain.org

Composition (INVE Products) Live food aquaculture training course www.aquatrain.org

Composition (RICH Products) Live food aquaculture training course www.aquatrain.org

Comparison Selco & RICH Live food aquaculture training course www.aquatrain.org

Comparison of Algae Rich and CS3000 Live food aquaculture training course www.aquatrain.org

Enrichment of rotifers with RICH Live food aquaculture training course www.aquatrain.org

Comparison RICH Advanced, Top & S.S. Live food aquaculture training course www.aquatrain.org