Bacterial Biofilms: Building Slimy Cities Gillian Ryan St. Francis Xavier University
Outline: Background The Model Preliminary Results Future plans
Biofilm: Community of microorganisms, including bacteria, fungi, and protozoa, found in aqueous environments Exopolysaccharides Division Chemotaxis Quorum Sensing
Biofilm: Picture of a cross sectional slice of a biofilm, taken with a laser scanning confocal microscope. Grown in our lab at StFX
The Model:
Base Forces i j r ij - Preserve Linear Momentum If rij Ro :
Quorum Sensing: On/off switch Controls EPS production Dynamically evaluated
Only produced by biofilm bacteria Amount of EPS emitted is a constant EPS spring force binds bacteria to EPS carrying water and EPS- carrying water to EPS-carrying water EPS:
Attractants: Amount of positive chemotactic signals emitted inversely related on number of bacterial neighbors Carried by water, which attracts bacteria Force is proportional to amount of attractant in water F A1 F A2
Division & Inhibitors: Spring Force holds dividing bacteria components together Inhibitory emissions dependent on bacterial neighbors Probability of division depends on inhibitor concentration
The Simulation Method: Molecular Dynamics, specifically Dissipative Particle Dynamics (DPD) GOOD FOR WATER FLOW ! Velocity-Verlet Algorithm
The Simulation: 1000 water spheres and 1 free bacterium World is initially a 10x10x10 micron box with periodic boundary conditions System is initialized with zero linear momentum
X X Y YZ Z Results: 1000 water 28 bacteria Healthy Planktonic Bacteria Healthy Biofilm Bacteria Inhibited Bacteria Water
X X Y YZ Z Results: 1000 water 99 bacteria Healthy Planktonic Bacteria Healthy Biofilm Bacteria Inhibited Bacteria Water
COMING SOON: SURFACES!
Acknowledgements: David Pink Bonnie Quinn Laura Filion Ryan MacDougall NSERC