Supplementary figure 1 Colorimetric analysis of inorganic P by molybdenium staining of hydrochloric extracts of rice grains in rice grains (non-embryo.

Slides:

Advertisements

Similar presentations

T-DNA Mutagenesis T-DNA Mutagenesis. Transfer-DNA Mutagenesis: a chemical or physical treatment that creates changes in DNA sequence which can lead to.

Advertisements

 V G SHOBHANA  Dr. N SENTHIL  KALPANA K.  Dr. P NAGARAJAN  Dr. M RAVEENDRAN  Dr. P BALASUBRAMANIAN CENTRE FOR PLANT MOLECULAR BIOLOGY TAMIL NADU.

What are the Methods and Approaches Used to Identify and Study Arabidopsis Seed Knock- Out Mutations? Eric Newton Garen Polatoglu Rena Schweizer.

At5g A mutant phenotype? Emily Eder HC70AL - Spring 2005.

Mutations in Arabidopsis Exocyst Gene AtSEC8 Jennie Hines Mentor: John Fowler.

Lecture 18, Chapter 11 Analysis of transgenic plants part I Mat Halter 3/27/12 Plant Genetics, Breeding and Biotechnology (PLSC 452/552), University of.

Lecture 19, Chapter 11 Analysis of transgenic plants part II Neal Stewart.

Analysis of Transgenic Plants. 1.Regeneration on Selective Medium Selectable Marker Gene.

Mapping in Arabidopsis Jeff Long Salk Institute. Cotyledons (seed leaves) Shoot Apical Meristem Hypocotyl (seedling stem) Root Root Apical Meristem.

(a) (b) (c) (d) (e) (f) (g) Figure S1.. Figure S1. Comparison of OsPCR1-6 and GW2 transcript levels in the grains of developing gw2 and wild-type isogenic.

Fig. S1 Mass spectra analysis of XXT4 reaction products demonstrating xylosyltransferase activity towards cellohexaose substrate. Predominant peaks represent.

Figure S1. Alignment of identified AtMYB93/92/53 homologues in land plants, used to infer the phylogeny in Figure 1B. Supporting information Figs S1-S7.

Figure S1. Figure S1. Relative expression levels of YUC family member transcripts in roots under high and low N conditions. Seedlings 4 days after germination.

AtPAT10 TIP1 Akr1p1 Akr2p Erf2p Swf1p Pfa5 Pfa3 GODZ HIP14 Pfa4 AtPAT10 TIP1 Akr1p1 Akr2p Erf2p Swf1p Pfa5 Pfa3 GODZ HIP14 Pfa4 AtPAT10 TIP1 Akr1p1 Akr2p.

THE FUNCTION OF AT5G04410 (NAC2) AND AT3G10500 (NAC2-like) NAC Family

Daisy Robinton Matt Emmer Jason Chai

Wsmutant a b Fig. S1 Morphology of the low-iron-sensitive mutant of Arabidopsis. (a) Wild type (Ws) and the low-iron-sensitive mutant (mutant) germinated.

Determining Functionality of Arabidopsis Thaliana Genes in Embryo Development Ria Yagnik.

Searching for Genes Important in Seed Development At1g19000 At1g74840 BY: Mike Douglas.

Arabidopsis Thaliana A Study of Genes and Embryo Development By Garen Polatoglu.

Is My Gene Important for Seed Development in Plants?? Gene: AT3G53370 Jonathan Milgrom Spring 2004.

Plants and algae Fungi Metazoa Protist Supplementary Figure 1. Phylogenetic analysis of GlsA1/ZRF orthologs in different organisms. Coloured background.

Similarities Cells-Nucleus- Chromosomes- DNA- genes Cells-Nucleus-Chromosomes- DNA- genes They are all located in cells.

WTPGMSF1 +15%PGMS G C C G C CG G Figure S1 Heteroduplex types and contents of PGMS gene amplicons with pre-PCR addition of 15% MT DNA. The.

Supplemental Figure 1. The cell death phenotype of fhy3 far1 double mutants. A. The cell death phenotype of fhy3-4 far1-2 mutant plants under LD conditions.

Volume 5, Issue 2, Pages (March 2012)

a b LB T-DNA RB Par WT PAR1/LAT4 Actin2 5’UTR 3’UTR At1g31830 Intron c

Volume 2, Issue 5, Pages (September 2009)

Tandem Inserts, Phenotypic Segregation, Hypocotyl Length, and More…

Supplemental Figure 2. (A) AtplaIVA-1 and AtplaIVA-2 null transcription lines for AtPLAIVA mRNA. RNAs from the relevant wild type Col were isolated.

Supplementary Figure 1 A B wild type ΔacsS

The Mitochondrion-Targeted PENTATRICOPEPTIDE REPEAT78 Protein Is Required for nad5 Mature mRNA Stability and Seed Development in Maize Ya-Feng Zhang,

The Alfin-like PHD Zinc Finger Transcription Factor Family

محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)

(x 10-3) (x 10-4) GST P WT mock WT #15 WT mock WT #15

PROGINS receptor gene polymorphism is associated with endometriosis

Consequences of T‐DNA insertion on SWP expression in swp mutant.

Volume 1, Issue 3, Pages (May 2008)

Volume 10, Issue 3, Pages (March 2017)

WT Segregant * Ds * * Figure S1. Fatty acid methyl esters of seed triacylglycerols for a wild-type segregant (WT Segregant) and transposon-tagged line.

Volume 6, Issue 6, Pages (November 2013)

Volume 4, Issue 1, Pages (January 2011)

Volume 1, Issue 2, Pages (March 2008)

Arabidopsis Transcription Factor Genes NF-YA1, 5, 6, and 9 Play Redundant Roles in Male Gametogenesis, Embryogenesis, and Seed Development Jinye Mu,

A Dual-Function Transcription Factor, AtYY1, Is a Novel Negative Regulator of the Arabidopsis ABA Response Network Tian Li, Xiu-Yun Wu, Hui Li, Jian-Hui.

Kim Min Jung , Ciani Silvano , Schachtman Daniel P. Molecular Plant

A Truncated Arabidopsis NUCLEOSOME ASSEMBLY PROTEIN 1, AtNAP1;3T, Alters Plant Growth Responses to Abscisic Acid and Salt in the Atnap1;3-2 Mutant Liu.

Osteopontin Gene is Expressed in the Dermal Papilla of Pelage Follicles in a Hair- Cycle-Dependent Manner Tian Yang, Pamela J. Jensen, Robert M. Lavker

Volume 2, Issue 5, Pages (September 2009)

Volume 2, Issue 1, Pages (January 2009)

Volume 8, Issue 5, Pages (May 2015)

Volume 7, Issue 1, Pages (January 2014)

A DTX/MATE-Type Transporter Facilitates Abscisic Acid Efflux and Modulates ABA Sensitivity and Drought Tolerance in Arabidopsis Haiwen Zhang, Huifen.

Rodríguez-Milla Miguel A. , Salinas Julio Molecular Plant

FLS2 Molecular Cell Volume 5, Issue 6, Pages (June 2000)

Both E12 and E47 Allow Commitment to the B Cell Lineage

Volume 2, Issue 1, Pages (January 2009)

Volume 5, Issue 1, Pages (January 2012)

Volume 5, Issue 4, Pages (July 2012)

The Arabidopsis Transcription Factor AtTCP15 Regulates Endoreduplication by Modulating Expression of Key Cell-cycle Genes Li Zi-Yu , Li Bin , Dong Ai-Wu.

Volume 10, Issue 1, Pages (January 2017)

Volume 5, Issue 6, Pages (November 2012)

MAB1 encodes a mitochondrial PDC E1β.

Supplementary Figure S1

Map-based cloning of ASI4 gene

Volume 2, Issue 4, Pages (July 2009)

Volume 4, Issue 4, Pages (July 2011)

Arabidopsis RPA2: A Genetic Link among Transcriptional Gene Silencing, DNA Repair, and DNA Replication Taline Elmayan, Florence Proux, Hervé Vaucheret

Volume 5, Issue 1, Pages (January 2012)

Volume 5, Issue 3, Pages (May 2012)

Presentation transcript:

Supplementary figure 1 Colorimetric analysis of inorganic P by molybdenium staining of hydrochloric extracts of rice grains in rice grains (non-embryo half). Left: 1A to 1D and 7E to 7H: P standards at amounts of 0.155μg, 0.465μg, 0.93μg, and 1.395μg, respectively; 2A to 2H: Os-lpa-XS110-1; 3A to 3H: Os- lpa-XS110-2; 4A to 4H: heterozygous Os-lpa-XS110-3; 5A to 5E and 6A to 6E: T 1 seeds of T-DNA activation tagging line 4A-02500; 7A to 7D: Dongjin (parental variety of 4A ). Right: 8A-8E, P standards at amounts of 0.00, 0.155μg, 0.465μg, 0.93μg, and 1.395μg, respectively; 9A to 9H: XS110; 10A to 10H: Os-lpa-XS110-2; 11A to 11H: homozygous Os-lpa-XS110-3; 12A to 12H, 13A to 13H, 14A to 14H, 15A to 15H, 16A to 16H and 17A to 17H; 18A to 18H, 19A to 19H: T 2 seeds from individual T 1 plants of 4A at T 2 generation.

Os-lpa-XS110-3 Xiushui retention time [min] response [mV] response [mV] InsP response [mV] InsP 6 Os-lpa-XS110-2 Standard inositol phosphates Supplementary figure 2. HPLC of acid-soluble Ins phosphates in wild type Xiushui 110 and its two LPA mutants. InsP 6 is hexakisphosphate, or phytic acid. InsP 3, InsP 4, and InsP 5 are tri-, tetrakis- and pentakisphosphates, respectively.

WT M LPA RSBRSB WT M LPA RSBRSB Actin MRP5 bp Supplementary figure 3. RT-PCR analysis of OsMRP5 gene using total RNA isolated from root (R), sheath (S) and blade (B) of 14-day old seedlings. M: DNA marker; WT: parent variety ‘Dongjing’; LPA: seedlings of low phytic acid seeds of T-DNA insertion lines at T 3 generation. RT-PCR was performed different tissues of. The actin gene was used as control.