DALIA KAMAL ELDIEN MOHAMMED Swab culture practical NO-5-

Case study A 6-year-old male was brought to the emergency department after suffering burns to the right arm when a hot cup of coffee spilled on him. presented with bilateral partial thickness burns to the arm representing approximately 10% total body surface area (TBSA). The burn on the right arm was red, partial thickness wounds were pusy, with change of the skin color, and cellulitis, determine the causative micro-organisms, and antimicrobial therapy

Technique of collection  Cleanse wound by removing excess debris from wound base with normal saline  Thoroughly flush wound with sterile saline.  Without touching swab to surrounding wound edges or skin, rotate tip of swab over a 1 cm area at the center of the open wound for 5 seconds  Place swab in culture transport sleeve.  Label culture collection/transport kit with name, birth date, specimen source, date and time of culture.  Place in lab biohazard transport bag.

Technique of culture  Remove protective cap from culture medium and insert cotton tipped applicator into the culture medium without contaminating the applicator shaft  The streaking is done using a sterile an inoculation loop.  The sample is spread across one quadrant of a petri dish containing a growth medium, usually an agar plate which has been sterilized in an autoclave  Sterilize the loop in the flame.

 Turn the plate 90 degrees and drag the steril loop through the area you have just streaked two to three times and continue to drag the loop in a "zig- zag" formation in the remaining half of the plate without touching that area again (area A).  Sterilize the loop in the flame.  Turn the plate 90 degrees. Repeat the procedure to make area B, C& zigzag  being very careful not to touch any of the areas you previously streaked