First Annual Meeting

Pioneering the Use of Genomic Biomarkers in Assessing Risk to Environmental and Human Health An established and effective collaboration A well-established need Particular local strengths:  model systems  high throughput approaches  toxicology Strategy and goals

The Indiana University Consortium for Environmental Genomics and Toxicology The Center for Genomics and Bioinformatics (IUB) The Center for Environmental Health (IUSOM) The School of Public and Environmental Affairs The Center for Research in Environmental Science (IUB)

Treaated labeled target Control labeled target RT Treated mRNA RT Control mRNA Contro l Treatment Microarray response data High-throughput experiments catalog all the genetic responses to drugs:

The summary is a signature of a drug’s effects. A gene expression signature of androgen stimulation was defined from gene expression profiles of LNCaP cells stimulated with the synthetic androgen R1881 for 12 hr and 24 hr, as compared to androgen-deprived LNCaP cells. The 27 gene signature contains both androgen- induced and androgen-repressed genes, shown here by row-normalized heat map.

A Strategy: High throughput water quality testing using gene expression signatures in Daphnia Stressed labeled target Control labeled target RT Stress mRNA RT Control mRNA Contr ol Environmental stress Gene response data Test stressors in the lab Make a catalog of signatures Use those signatures to guide toxicological work in mammals Look for those signatures to assess water quality

The Results: Assessment – Sensitivity and information- rich measurement of "bio-active" titers. Diagnosis – Enhanced understanding of the mechanisms of toxicity and a better assessment of risk. Combining the evaluation of human and ecological risks. The Goal: New regulatory standards that take advantage of these inexpensive, information-rich approaches.

Dr. John Colbourne Associate Scientist Genomics Director, CGB (IUB) Barbara Hocevar Assistant Professor Department of Pharmacology and Toxicology School of Medicine (IUPUI) Peter Cherbas Professor Biology Director, CGB (IUB) James Klaunig Robert B. Forney Professor Dept. of Pharmacology and Toxicology Director, Center for Environmental Health School of Medicine (IUPUI) Lisa Kamendulis Assistant Professor Department of Pharmacology and Toxicology School of Medicine (IUPUI) Keith Clay Professor of Biology Director, IU Research and Teaching Preserve Director, CRES (IUB) Joseph Shaw Assistant Professor School of Public and Environmental Affairs (IUB) The Indiana University Consortium for Environmental Genomics and Toxicology

First Annual Meeting