1.What do you think the word bioenergetics means? Why? 2.Infer how bioenergetics relates to muscles.

Fuel for Exercise: Bioenergetics and Muscle Metabolism

Terminology Substrates –Fuel sources from which we make energy (adenosine triphosphate [ATP]) –Carbohydrate, fat, protein Bioenergetics –Process of converting substrates into energy –Performed at cellular level Metabolism: chemical reactions in the body

Measuring Energy Release Can be calculated from heat produced 1 calorie (cal) = heat energy required to raise 1 g of water from 14.5°C to 15.5°C 1,000 cal = 1 kcal = 1 Calorie (dietary)

Substrates: Fuel for Exercise Carbohydrate, fat, protein –Carbon, hydrogen, oxygen, nitrogen Energy from chemical bonds in food stored in high-energy compound ATP Resting: 50% carbohydrate, 50% fat Exercise (short): more carbohydrate Exercise (long): carbohydrate, fat

Carbohydrate All carbohydrate converted to glucose –4.1 kcal/g; ~2,500 kcal stored in body –Primary ATP substrate for muscles, brain –Extra glucose stored as glycogen in liver, muscles Glycogen converted back to glucose when needed to make more ATP Glycogen stores limited (2,500 kcal), must rely on dietary carbohydrate to replenish

Fat Efficient substrate, efficient storage –9.4 kcal/g –+70,000 kcal stored in body Energy substrate for prolonged, less intense exercise –High net ATP yield but slow ATP production –Must be broken down into free fatty acids (FFAs) and glycerol –Only FFAs are used to make ATP

Protein Energy substrate during starvation –4.1 kcal/g –Must be converted into glucose (gluconeogenesis) Can also convert into FFAs (lipogenesis) –For energy storage –For cellular energy substrate

1.What is going to give off more energy? Fats or carbohydrates? Why? 2.What is harder to break down proteins or fat?

Figure 2.1

Controlling Rate of Energy Production by Substrate Availability Energy released at a controlled rate based on availability of primary substrate Mass action effect –Substrate availability affects metabolic rate –More available substrate = higher pathway activity –Excess of given substrate = cells rely on that energy substrate more than others

Controlling Rate of Energy Production by Enzyme Activity Energy released at a controlled rate based on enzyme activity in metabolic pathway Enzymes –Do not start chemical reactions or set ATP yield –Do facilitate breakdown (catabolism) of substrates –Lower the activation energy for a chemical reaction –End with suffix -ase ATP broken down by ATPase

Figure 2.2

Controlling Rate of Energy Production by Enzyme Activity Each step in a biochemical pathway requires specific enzyme(s) More enzyme activity = more product Rate-limiting enzyme –Can create bottleneck at an early step –Activity influenced by negative feedback –Slows overall reaction, prevents runaway reaction

Figure 2.3

Stored Energy: High-Energy Phosphates ATP stored in small amounts until needed Breakdown of ATP to release energy –ATP + water + ATPase  ADP + P i + energy –ADP: lower-energy compound, less useful Synthesis of ATP from by-products –ADP + P i + energy  ATP (via phosphorylation) –Can occur in absence or presence of O 2

Figure 2.4

Bioenergetics: Basic Energy Systems ATP storage limited Body must constantly synthesize new ATP Three ATP synthesis pathways –ATP-PCr system (anaerobic metabolism) –Glycolytic system (anaerobic metabolism) –Oxidative system (aerobic metabolism)

1.What two substrates give off the same amount of energy? How much is it? 2.What does an enzyme do for us?

1.What is ATP made of? 2.Draw the structure.`

1.What are the three pathways we use to synthesize ATP? 2.Which are anaerobic and which are aerobic?

1.Write the equation for the breakdown of ATP. 2.What is ATPase? Using your answer explain what it does in a chemical reaction.

1.Write down one similarity and one difference we found on Friday. 2.When do you think these different energy sources are used?

Three ways to create ATP ATP-PC (high power, short duration) Glycolytic (moderate power/short duration) Oxidative (low power/long duration).

ATP-PCr System Anaerobic, substrate-level metabolism ATP yield: 1 mol ATP/1 mol PCr Duration: 3 to 15 s Because ATP stores are very limited, this pathway is used to reassemble ATP

ATP-PCr System Phosphocreatine (PCr): ATP recycling –PCr + creatine kinase  Cr + P i + energy –PCr energy can be used to reassemble ATP Replenishes ATP stores during rest Recycles ATP during exercise until used up (~3-15 s maximal exercise) Examples: a short sprint, a punch or kick, or pitching a baseball.

Figure 2.5

Control of ATP-PCr System: Creatine Kinase (CK) PCr breakdown catalyzed by CK CK controls rate of ATP production –Negative feedback system –When ATP levels  (ADP  ), CK activity  –When ATP levels , CK activity 

Glycolytic System Anaerobic ATP yield: 2 to 3 mol ATP/1 mol substrate Duration: 15 s to 2 min Breakdown of glucose via glycolysis Examples: any moderately-long runs such as yards, a 1:30 effort of all-out MMA maneuvers, or a one-minute full-court press - offense display - and another full-court press effort in basketball.

Glycolytic System Uses glucose or glycogen as its substrate –Costs 1 ATP for glucose, 0 ATP for glycogen Pathway starts with glucose, ends with pyruvic acid –All steps occur in cytoplasm –ATP yield: 2 ATP for glucose, 3 ATP for glycogen

Glycolytic System Cons –Low ATP yield, inefficient use of substrate –Lack of O 2 converts pyruvic acid to lactic acid Pros –Allows muscles to contract when O 2 is limited –Permits shorter-term, higher-intensity exercise than oxidative metabolism can sustain

Glycolytic System Phosphofructokinase (PFK) –Rate-limiting enzyme  ATP (  ADP)   PFK activity  ATP   PFK activity –Also regulated by products of Krebs cycle Glycolysis = ~2 min maximal exercise Need another pathway for longer durations

Oxidative System Aerobic ATP yield: depends on substrate –32 to 33 ATP/1 glucose –100+ ATP/1 FFA Duration: steady supply for hours Most complex of three bioenergetic systems Occurs in the mitochondria, not cytoplasm

Oxidation of Carbohydrate Stage 1: Glycolysis Stage 2: Krebs cycle Stage 3: Electron transport chain

Oxidation of Carbohydrate: ATP yield same as anaerobic glycolysis Same general steps as anaerobic glycolysis but, in the presence of oxygen, Pyruvic acid  acetyl-CoA, enters Krebs cycle

Oxidation of Carbohydrate: Energy Yield 1 glucose = 32 ATP 1 glycogen = 33 ATP Breakdown of net totals –Glycolysis = +2 (or +3) ATP –GTP from Krebs cycle = +2 ATP –10 NADH = +25 ATP –2 FADH = +3 ATP

Oxidation of Fat Triglycerides: major fat energy source –Broken down to 1 glycerol + 3 FFAs –Lipolysis, carried out by lipases Rate of FFA entry into muscle depends on concentration gradient Yields ~3 to 4 times more ATP than glucose Slower than glucose oxidation

Oxidation of Protein Rarely used as a substrate –Starvation –Can be converted to glucose (gluconeogenesis) –Can be converted to acetyl-CoA Energy yield not easy to determine –Nitrogen presence unique –Generally minimal

Control of Oxidative Phosphorylation: Negative Feedback Negative feedback regulates Krebs cycle Isocitrate dehydrogenase: rate- limiting enzyme –Similar to PFK for glycolysis –Inhibited by ATP, activated by ADP

Figure 2.11

Figure 2.9

Figure 2.10

Figure 2.12

Interaction Among Energy Systems All three systems interact for all activities –No one system contributes 100%, but –One system often dominates for a given task More cooperation during transition periods

Figure 2.13

Oxidative Capacity of Muscle Not all muscles exhibit maximal oxidative capabilities Factors that determine oxidative capacity –Enzyme activity –Fiber type composition, endurance training –O 2 availability versus O 2 need

Enzyme Activity Not all muscles exhibit optimal activity of oxidative enzymes Enzyme activity predicts oxidative potential Representative enzymes –Succinate dehydrogenase, the more of this the more endurance. 2-4 times more in endurance runners. Endurance trained versus untrained

Figure 2.14

Fiber Type Composition and Endurance Training Type I fibers: greater oxidative capacity –More mitochondria –High oxidative enzyme concentrations –Type II better for glycolytic energy production Endurance training –Enhances oxidative capacity of type II fibers –Develops more (and larger) mitochondria

Oxygen Needs of Muscle As intensity , so does ATP demand In response –Rate of oxidative ATP production  –O 2 intake at lungs  –O 2 delivery by heart, vessels  O 2 storage limited—use it or lose it O 2 levels entering and leaving the lungs accurate estimate of O 2 use in muscle