DNA Replication Copying the genetic code
DNA Replication Unpack the Chromosomes Unzip the DNA Base pairing at the replication fork Leading strand vs Lagging strand
Making Copies
copycopied during cell division Sister Chromatids (identical copies)
Accessing the DNA inactive DNA (deactivated or mitotic) DNA in use
Visualization of DNA Packing For this video, visit: /DNAi_packaging_vo2.html /DNAi_packaging_vo2.html /DNAi_packaging_vo2.html
Accessing the DNA
DNA Structure
Complementary Base Pairing
Identical Parent and Daughter Strands
Replication Bubbles helicase 5’ 3’ 5’
Replication Bubbles 5’ 3’ 5’
Replication Bubbles 5’ 3’ 5’
Replication Bubbles copies Replication Fork 5’ 3’ 5’
Replication Bubbles
Releasing the Tension
Topoisomerase (gyrase) cleaves and uncoils DNA ahead of helicase Single-stranded DNA binding proteins (SSB) prevent DNA from coiling back up
DNA Replication Primase RNA primer DNA Polymerase III 5’ 3’ 5’
DNA Replication The leading strands grow toward the replication fork PROBLEM: DNA polymerase III only works in the 5’-3’ direction. Leading Strands 5’ 3’ 5’
DNA Replication 5’ 3’ 5’
DNA Replication Lagging Strands The lagging strands grow away from the replication fork 5’ 3’ 5’
DNA Replication 5’ 3’ 5’ DNA Polymerase I DNA Ligase
DNA Replication 5’ 3’ 5’
DNA Replication continuous & discontinuous Okazaki Fragments 5’ 3’ 5’ DNA Polymerase moves in a 5’ - 3’ direction 5’-3’ chain synthesized continuously 3’-5’ chain synthesized discontinuously Results in a series of Okazaki fragments Later sealed up by DNA ligase
The Whole Process in 3D For this video, visit: /DNAi_replication_schematic.html /DNAi_replication_schematic.html /DNAi_replication_schematic.html
The Whole Process in 3D For this video, visit: DNAi_replication_vo2.html DNAi_replication_vo2.html
Animations DNA Replication: McGraw-Hill: DNA Replication & Meselson-Stahl: hill.com/sites/ /student_view0/chapter14/ animations.html# hill.com/sites/ /student_view0/chapter14/ animations.html# hill.com/sites/ /student_view0/chapter14/ animations.html# DNA Song:
Credits Molecule drawing created using ChemSketch. Some from the Pearson or Nelson Biology 12 textbook. Some images from Wikimedia Commons Some from unknown sources.