Metabolomics and analytical chemistry: GC & GCMS Simone Bossi Analytical Chemistry Lab – Plant Physiology – Plant Biology.

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Metabolomics and analytical chemistry: GC & GCMS Simone Bossi Analytical Chemistry Lab – Plant Physiology – Plant Biology

Metabolomics is the "systematic study of the unique chemical fingerprints that specific cellular processes leave behind" - specifically, the study of their small- molecule metabolite profiles. B. Daviss, "Growing pains for metabolomics," The Scientist, 19[8]:25-28, April 25, 2005 Metabolome Proteome Transcriptome Genome

Analytical methods able to qualify and quantify single molecules or a class of metabolites. Colorimetry, Spectrophotometry, Electrophoresys, Immunology, Radiometry, Mass Spectrometry, NMR, Crystallography,…

CHROMATOGRAPHY Separate complex mixture coming from biological sample. CLASSIFICATION Mobile phase, stationary phase classification Shape of the support on which the stationary phase is fixed Operating conditions Chemistry or stechiometry of molecules with the phases …

Gas chromatography Volatile organic compounds Hydrophobic molecules extracted or dissolved in organic solvents Small molecules weakly polar Big and hydrophobic molecules volatile at high temperature

Liquid Injection

SPME: Solid Phase Micro Extraction

SBSE: Stir Bar Sorptive Extraction

The Gas chromatographer

The Injection port

The Column

The Oven

Data Analysis

Mass Spectrum and Identification

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